Behavioural compatibility, not fear, best predicts the looking patterns of chacma baboons.

Animal vigilance is often investigated under a narrow set of scenarios, but this approach may overestimate its contribution to animal lives. A solution may be to sample all looking behaviours and investigate numerous competing hypotheses in a single analysis. In this study, using a wild group of habituated chacma baboons (Papio ursinus griseipes) as a model system, we implemented a framework for predicting the key drivers of looking by comparing the strength of a full array of biological hypotheses.

Encapsulation of Transketolase into -Assembled Protein Nanocompartments Improves Thermal Stability.

Protein compartments offer definitive structures with a large potential design space that are of particular interest for green chemistry and therapeutic applications. One family of protein compartments, encapsulins, are simple prokaryotic nanocompartments that self-assemble from a single monomer into selectively permeable cages of between 18 and 42 nm. Over the past decade, encapsulins have been developed for a diverse application portfolio utilizing their defined cargo loading mechanisms and repetitive surface display.

Fine-tuning the activity and stability of an evolved enzyme active-site through noncanonical amino-acids.

Site-specific saturation mutagenesis within enzyme active sites can radically alter reaction specificity, though often with a trade-off in stability. Extending saturation mutagenesis with a range of noncanonical amino acids (ncAA) potentially increases the ability to improve activity and stability simultaneously. Previously, an Escherichia coli transketolase variant (S385Y/D469T/R520Q) was evolved to accept aromatic aldehydes not converted by wild-type.

The Two-Species Model of transketolase explains donor substrate-binding, inhibition and heat-activation.

We recently characterised a low-activity form of E. coli transketolase, TK, which also binds the cofactor thiamine pyrophosphate (TPP) with an affinity up to two-orders of magnitude lower than the previously known high TPP-affinity and high-activity form, TK, in the presence of Mg. We observed previously that partial oxidation was responsible for increased TK activity, while low-activity TK was unmodified.

Novel insights into transketolase activation by cofactor binding identifies two native species subpopulations.

Transketolase (TK) cofactor binding has been studied extensively over many years, yet certain mysteries remain, such as a lack of consensus on the cooperativity of thiamine pyrophosphate (TPP) binding into the two active sites, in the presence and absence of the divalent cation, Mg. Using a novel fluorescence-based assay, we determined directly the dissociation constants and cooperativity of TPP binding and provide the first comprehensive study over a broad range of cofactor concentrations. We confirmed the high-affinity dissociation constants and revealed a dependence of both the affinity and cooperativity of binding on [Mg], which explained the previous lack of consensus.

Improving anaerobic digestion of a cellulosic waste via routine bioaugmentation with cellulolytic microorganisms.

This study investigated routine bioaugmentation in the acid-phase of a two-phase anaerobic digestion (AD) process treating a largely cellulosic waste material generated from sweet corn processing. A proprietary cellulolytic bioculture was used for bioaugmentation with the aim of increasing substrate hydrolysis to improve overall methanogenic efficiency. In a sequencing batch experiment routine bioaugmentation achieved significantly greater soluble chemical oxygen demand (sCOD) generation (+25%) and methane production (+15%) compared to one-time bioaugmentation.

The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis.

Correct cell fate choice is crucial in development. In post-embryonic development of the hermaphroditic Caenorhabitis elegans, distinct cell fates must be adopted in two diverse tissues. In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes.