Discerning assimilated diets of wild animals using stable isotopes is well established where potential dietary items in food webs are isotopically distinct. With the advent of mixing models, and Bayesian extensions of such models (Bayesian Stable Isotope Mixing Models, BSIMMs), statistical techniques available for these efforts have been rapidly increasing. The accuracy with which BSIMMs quantify diet, however, depends on several factors including uncertainty in tissue discrimination factors (TDFs; Δ) and identification of appropriate error structures.
View Article and Find Full Text PDFExogenous enzymes as alternatives to feed antibiotics in poultry has become an emerging research area with the emergence of antibiotic resistance. The objective was to evaluate the effects of diet medication (antibiotics) and β-glucanase (BGase) on digesta soluble β-glucan depolymerization, carbohydrate fermentation, and performance of coccidiosis-vaccinated broiler chickens fed wheat-based diets. A total of 1,782 broilers were raised on litter floor pens, and each treatment was assigned to 1 pen in each of the 9 rooms.
View Article and Find Full Text PDFExogenous β-glucanase (BGase) improves nutrient digestibility and production performance in laying hens fed barley-based diets, but the effect of enzyme and the dosage on β-glucan depolymerization and fermentation in the gastrointestinal tract is poorly understood. The objectives of the study were to determine the effects of hulless barley (HB) and BGase levels on digestive tract β-glucan depolymerization and fermentation in laying hens. A total of 108 Lohman-LSL Lite hens were housed in cages and fed 2 levels of HB (CDC Fibar; 0 and 73%) by substituting wheat in the diet and graded levels of BGase (Econase GT 200 P from ABVista; 0, 0.
View Article and Find Full Text PDFExogenous β-glucanase (BGase) in barley-based feed has been shown to reduce digesta viscosity in chickens, and thereby improve performance. Less well studied is the potential for BGase to convert barley β-glucan into low molecular weight carbohydrates, which might influence digestive tract function and enteric disease. Coccidiosis-vaccinated broiler chickens were fed graded levels of hulless barley (HB) and BGase to determine their effects on β-glucan depolymerization and digestive tract characteristics.
View Article and Find Full Text PDFPLoS One
October 2021
Introduction: Limited use of medication in poultry feed led to the investigation of exogenous enzymes as antibiotic alternatives for controlling enteric disease. The objective of this study was to evaluate the effects of diet β-glucanase (BGase) and medication on β-glucan depolymerization, digestive tract characteristics, and growth performance of broilers.
Materials And Methods: Broilers were fed hulless barley (HB) based diets with BGase (Econase GT 200P from AB Vista; 0 and 0.
The reduced use of antibiotics in poultry feed has led to the investigation of alternatives to antibiotics, and one such substitution is fermentable carbohydrates. Exogenous β-glucanase (BGase) is commonly used in poultry fed barley-based diets to reduce digesta viscosity. The effects of hulless barley (HB) and BGase levels on ileal digesta soluble β-glucan molecular weight, digestive tract characteristics, and performance of broiler chickens were determined.
View Article and Find Full Text PDFDiet β-glucanase (BGase) depolymerizes viscous β-glucan into lower molecular weight carbohydrates, which might act as a prebiotic in chickens exposed to enteric disease. Coccidiosis-challenged broiler chickens were fed graded levels of hulless barley (HB) and BGase to determine their effects on growth performance. Broilers were fed high β-glucan HB (CDC Fibar; 0, 30, and 60% replacing wheat) and BGase (Econase GT 200P; 0, 0.
View Article and Find Full Text PDFProtein quality assessment of feed ingredients for poultry is often achieved using in vitro or in vivo testing. In vivo methods can be expensive and time consuming. Protein quality can also be evaluated using less expensive and time consuming chemical methods, termed in vitro.
View Article and Find Full Text PDFThe objective of this research was to evaluate activation of the ileal brake in broiler chickens using diets containing semi-purified wheat (WS; rapidly and highly digested) and pea (PS; slowly and poorly digested) starch. Diets were formulated to contain six WS:PS ratios (100:0, 80:20, 60:40, 40:60, 20:80, 0:100) and each starch ratio was fed to 236 Ross 308 male broilers housed in 4 litter floor pens. At 28 d of age, the effect of PS concentration was assessed on starch digestion, digestive tract morphology, and digesta pH and short-chain fatty acid (SCFA) concentration.
View Article and Find Full Text PDFThe effect of nutrition during the early life of turkey poults has a long-lasting impact on bird performance. This study assessed the digestibility of 5 high protein feed ingredients (soybean meal [SBM], corn gluten meal [CGM], canola protein concentrate [CPC], fish meal [FM], and porcine meal [PCM]) in broiler chickens, as well as their use in turkey pre-starter diets fed to 21 d of age. The first experiment (5 × 2 factorial arrangement) determined nitrogen corrected apparent metabolizable energy (AMEn) and apparent ileal amino acid digestibility (AIAAD) of each ingredient in broiler chickens at 5 and 21 d of age, using 6 replications of 30 and 8 chicks, respectively.
View Article and Find Full Text PDFIndigestible dietary protein fermentation products have been suggested to negatively influence broiler performance due to their impact on health and digestive tract morphology. This study evaluated the digestive tract morphology and caecal protein fermentation metabolites of broiler fed 3 dietary protein levels (24%, 26% and 28%) with low or high indigestible protein (LIP, HIP). Two completely randomized 3 × 2 factorial trials were conducted with protein level (PL) and indigestible protein (IDP) as the main factors.
View Article and Find Full Text PDFMyo-inositol (inositol) affects memory, and the incidence of depression and anxiety in mammals. An experiment was designed to determine if pure inositol (0.16%), or high levels of phytase (3000 FTU/kg) affect the behaviour of fully beaked Lohmann LSL lite hens fed amino acid sufficient (19% crude protein (CP)) and deficient diets (16% CP), from 19 to 59 weeks of age.
View Article and Find Full Text PDFGnotobiotic broiler chickens were used to study interactive effects of supplemented phosphorus, calcium (PCa), and phytase (Phy) on myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6) degradation and release of myo-inositol in the digestive tract. In 2 subsequent runs, the chickens were subjected to 1 of 4 dietary treatments with and without PCa and Phy supplementation. Sanitized eggs were hatched in 8 germfree isolators, and a minimum of 9 male Ross 308 chickens were placed in each pen (total 16 pens).
View Article and Find Full Text PDFThe inclusion of starch with a lower rate and extent of digestion has proven to be beneficial in broiler production. However, less is known about its effect on laying hen performance. Therefore, 6 diets were formulated to produce differing ratios (0:100, 20:80, 40:60, 60:40, 80:20, and 100:0) of semi-purified wheat starch (rapidly digested) and pea starch (PS; slowly digested).
View Article and Find Full Text PDFDietary starch with lower rate and extent of digestion improves broiler feed efficiency, but previous results might have been confounded by non-starch components of the grains. Therefore, the objective of this research was to study the effects of starch digestion on broilers using semi-purified starch. Semi-purified wheat (WS, rapidly digested) and pea (PS, slowly digested) starch were combined to create 6 WS:PS ratios (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100) in starter, grower and finisher diets.
View Article and Find Full Text PDFIt is difficult to obtain digestion kinetics data of high protein ingredients using chickens. Collecting kinetics data requires repeated sampling of digesta from the small intestine during the digestion process, which is not easily accomplished due to the anatomical structure of chicken digestive tract. An technique is proposed for measuring the digestion kinetics of protein sources fed to chickens.
View Article and Find Full Text PDFObjective: The objective of this study is to investigate the structural and functional ocular changes that develop in turkeys exposed to a photoperiod of 23 h of light (23L) compared with a photoperiod of 14 h of light (14L).
Procedures: Ten-day-old Nicholas heavy strain poults were exposed to either a 14L or 23L photoperiod. Between 16 and 18 weeks of age, equal numbers of turkeys per treatment group underwent ophthalmic examination (biomicroscopy, indirect ophthalmoscopy) (n = 14), refractometry (n = 20), keratometry (n = 20), tonometry (n = 20), and full-field electroretinography (ERG) (n = 14).
Daylength used as a management tool has powerful implications on the welfare of both broilers and turkeys. Near-constant light results in many detrimental impacts, including lack of behavioural rhythms and circadian melatonin rhythms. Both are suggestive that sleep fragmentation could result in birds reared on long photoperiods, which can lead to the same negative health and physiological responses as total sleep deprivation.
View Article and Find Full Text PDFBackground: Individuals engaged in work in intensive animal houses experience some of the highest rates of occupationally related respiratory symptoms. Organic dust and in particular endotoxin has been most closely associated with respiratory symptoms and lung function changes in workers. It has previously been shown that for intensive poultry operations, type of poultry housing [cage-housed (CH) versus floor-housed (FH)] can influence the levels of environmental contaminants.
View Article and Find Full Text PDFAvailability of gram quantities of purified soybean lectin (SBL) to scientists will foster discovery of novel biomedical applications of the lectin and provide the opportunity to investigate the antinutritional effects of SBL in soybean-consuming food animals and poultry. Therefore, a semi-pilot-scale procedure for isolating and purifying SBL was designed. Defatted soyflour was extracted overnight with 0.
View Article and Find Full Text PDFComp Biochem Physiol C Toxicol Pharmacol
July 2003
Luminal polyamines and their absorption are essential for proliferation of the enterocytes and, therefore, nutrition, health and development of the animal. The transport systems that facilitate the uptake of putrescine were characterized in chick duodenal, jejunal and ileal brush-border membrane vesicles prepared by MgCl2 precipitation from three-week-old chicks. An inwardly-directed Na+ gradient did not stimulate putrescine uptake and, therefore, putrescine transport in chick intestine.
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