Orbital shaking conditions augment human nasoseptal cartilage formation in 3D culture.

This study aimed to determine whether a dynamic orbital shaking culture system could enhance the cartilage production and viability of bioengineered nasoseptal cartilage. Human nasal chondrocytes were seeded onto nanocellulose-alginate biomaterials and cultured in static or dynamic conditions for 14 days. Quantitative polymerase chain reaction for chondrogenic gene expression (type 2 collagen, aggrecan and ) was performed, demonstrating a transient rise in expression at 1 and 7 days of culture, followed by a rise at 7 and 14 days in Aggrecan (184.