Publications by authors named "Henrique da Costa Oliveira"
Article Synopsis
- DNA is a highly efficient data storage medium, known for its stability and ability to store vast amounts of information compactly.
- Despite its advantages, challenges such as scalability, standardization, and security need to be addressed for broader adoption.
- Collaboration among experts is essential to harness the potential of DNA storage, which could lead to low energy costs and extremely durable data retention.
Despite the lack of biochemical information, all available in silico metabolic models of Pseudomonas putida KT2440 consider NADP as the only cofactor accepted by the glucose-6-phosphate dehydrogenases. Because the Entner-Doudoroff pathway is the main glycolytic route in this bacterium, determining how much NADH and NADPH are produced in the reaction catalyzed by these enzymes is very important for the correct interpretation of metabolic flux distributions. To determine the actual cofactor preference of the glucose-6-phosphate dehydrogenase encoded by the zwf-1 gene (PputG6PDH-1), the major isoform during growth on glucose, we purified this protein and studied its kinetic properties.
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