Semisolid culture medium improves mycelial recovery of Agaricus subrufescens cryopreserved in cereal grains.

This study aimed to evaluate the effects of the solid and semisolid culture medium on the mycelial viability of A. subrufescens after 5-year cryopreservation at - 70 °C. Mycelia were grown in three types of whole or ground grains, with or without 5% glycerol addition in the substrate and/or in a cryotube.

Cryopreservation at -20 and -70 °C of Pleurotus ostreatus on Grains.

Alternative substrates for cryopreservation at -20 °C have been little explored for basidiomycetes and could bring new possibilities of lower cost cryopreservation. Nevertheless, freezing temperatures between -15 and -60 °C are very challenging because they frequently result in cryoinjuries. The objective of this study was to evaluate substrates associated to cryoprotective agents for Pleurotus ostreatus cryopreservation at -20 or -70 °C in order to develop alternative techniques for basidiomycete cryopreservation.