The principle of pooled calibrations and outlier retainment elucidates optimum performance of ion chromatography.

A principle with quality assurance of ion chromatography (IC) is presented. Since the majority of scientists and costumers are interested in the determination of the true amount of analyte in real samples, the focus of attention should be directed towards the concept of accuracy rather than focussing on precision. By exploiting the principle of pooled calibrations and retainment of all outliers it was possible to obtain full correspondence between calibration uncertainty and repetition uncertainty, which for the first time evidences statistical control in experiments with ion chromatography.

Complexation of tauro- and glyco-conjugated bile salts with three neutral beta-CDs studied by ACE.

Complexation of the bile salts (BS) taurocholate, tauro-beta-muricholate, taurodeoxycholate, taurochenodeoxycholate, glycocholate, glycodeoxycholate, and glycochenodeoxycholate common in rat, dog, and man with natural beta-CD and the chemically modified beta-CDs 2-hydroxypropyl-beta-CD and 2-O-methyl-beta-CD was studied using mobility shift ACE. The CDs were selected due to their frequent use in preformulation and drug formulation as oral excipients for the solubilization of drug substances with low aqueous solubility. ACE was demonstrated to be a feasible and efficient technique for investigation of the interactions between BS and beta-CDs.