Publications by authors named "Henriette Toft Boesen"
The whole-blood interferon-gamma (IFN-γ) assay is a quantitative in vitro assay for a direct read-out of Ag-specific cell-mediated immune (CMI) responses to infectious diseases. The IFN-γ assay is robust in severe intracellular infections like Brucella or mycobacteria, but more difficult to evaluate for less severe or immunocompromising infections. Here we investigated the performance of the assay when recombinant co-stimulatory cytokines IL-12 and/or IL-18 were added along with Ag or PBS to cultures of whole-blood from pigs infected with Lawsonia intracellularis.
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- A new enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies against the porcine pathogen Lawsonia intracellularis, evaluated using serum from both naturally and experimentally infected pigs.
- The ELISA showed a sensitivity of 98.0% and a specificity of 99.3%, indicating high accuracy based on a substantial sample size.
- This method could serve as an effective alternative to current serological tests, aiding in the diagnosis of proliferative enteropathy and assessing herd health status regarding this pathogen.