Influence of daily oral prophylactic selenium treatment on the dibutyltin dichloride (DBTC)-induced pancreatitis in rats.

Dibutyltin dichloride (DBTC) is an organotin compound used as model for acute and chronic pancreatitis. Oxidative stress is one of the mechanisms of propagation of acinar cell injury in acute pancreatitis. Selenium is an essential cofactor in the antioxidant glutathione peroxidase pathway.

In vitro study of drug-eluting stent coatings based on poly(L-lactide) incorporating cyclosporine A - drug release, polymer degradation and mechanical integrity.

In this study, absorbable polymer stent coatings for localized drug delivery based on poly(L-lactide) (PLLA) and cyclosporine A (CsA) were developed and tested in vitro. Metallic stents were coated with different compositions of PLLA/CsA (70/30, 60/40, 50/50% w/w) and beta-sterilized. The specimens were used to assess the drug release kinetics with HPLC.

Influence of selenium treatment on the acute toxicity of dibutyltin dichloride in rats.

Background/aims: Dibutyltin dichloride (DBTC) is an organotin compound used as a model for acute pancreatitis. The aim was to determine the effect of various doses of Na-selenite on the pathogenesis and course of DBTC-induced toxic changes in organs and serum of rats.

Methods: Experimental pancreatitis was induced by intravenous administration of 6 mg kg(-1) BW DBTC.

Formation of methanol and formate in Wistar rats after oral administration of methylated rapeseed oil: a fuel for lamps.

Low viscosity, low surface tension and low volatility are features of lamp oils contributing to chemical pneumonia that can occur after ingestion. Because lamp oils with such physico-chemical properties have been forbidden in the European Community from July 2000 onward, industry has developed different products, mostly based upon rapeseed oil. The fatty acids of these oils are methylated.

Repeated administration of a mild acute toxic dose of di-n-butyltin dichloride at intervals of 3 weeks induces severe lesions in pancreas and liver of rats.

Di-n-butyltin dichloride (DBTC) induced thymus atrophy, bile duct lesions, pancreatitis, and liver lesions in rats. Depending on dose [6 and 8 mg/kg intravenous (i.v.

Influence of curcumin on cyclosporin-induced reduction of biliary bilirubin and cholesterol excretion and on biliary excretion of cyclosporin and its metabolites.

We investigated the ability of curcumin, which can be extracted from different Curcuma species, to prevent cyclosporin-induced reduction of biliary bilirubin and cholesterol excretion, and its influence on biliary excretion of cyclosporin (CS) and its metabolites in the bile fistula model in rats. I.v.

Antidotal effects of 2,3-dimercaptopropane-1-sulfonic acid (DMPS) and meso-2,3-dimercaptosuccinic acid (DMSA) on the organotoxicity of dibutyltin dichloride (DBTC) in rats.

1. Dialkyltin compounds have been widely used in industry and agriculture, mainly as biocides, catalysts and plast stabilizer. In dependence on the length of the alkyl chains these organotins exert toxic effects on the immune system, the bile duct, liver and pancreas.

The course of pancreatic fibrosis induced by dibutyltin dichloride (DBTC).

In summary, in addition to an acute interstitial pancreatitis the organotin compound DBTC induced a pancreatic fibrosis in rats. The course of the pancreatic fibrosis was studied 2-36 weeks after single i.v.

The influence of ethanol on long-term effects of dibutyltin dichloride (DBTC) in pancreas and liver of rats.

The present study was done to determine the additional influence of daily ethanol intake (15% in drinking water ad libitum) on long-term toxic effects of a single administration of dibutyltin dichloride (DBTC, 8 mg/kg b.w. i.

Acute interstitial pancreatitis in rats induced by dibutyltin dichloride (DBTC): pathogenesis and natural course of lesions.

Dibutyltin dichloride (DBTC; 6 mg/kg body weight, i.v.) induced acute interstitial pancreatitis in rats.

[The effect of barbiturates and diphenylhydantoin on the mitotic activity of thymocytes and bone marrow cells in mice and rats].

Phenobarbitone (PB) and diphenylhydantoin (DPH) inhibited the mitotic rate of thymocytes and bone marrow cells (colchicine blocked metaphases) dose- and time-dependent (20-80 mg/kg i.p., 12-48 hours) in mice and rats in vivo.

Acute pancreatitis and bile duct lesions in rat induced by dibutyltin dichloride.

After i.v. or i.

[Seasonal variations of the mitotic activity of thymocytes in CBA mice].

Considerable seasonal variations were exhibited by mitotic activities of thymocytes in CBA mice. In April and May they were about twice as high as in October and November. Biorhythmic changes in mitotic activity of thymocytes should be taken into consideration for any study of drug effects on the thymus.

[Biological testing of biomaterials in the framework of permitted procedures. 2].

Submitted is the revised version of a proposal for a guideline for biological testing of materials (biomaterials) which remain temporarily or permanently, resp., at or in the human body or have contact with drugs or body fluids outside the human body.

[Effects of dithiocarbamates on the mitotic activity of thymocytes].

The effects of dithiocarbamates on the mitotic activity of thymocytes were studied in vitro and in vivo. The mitotic activity was determined by counting of the metaphases blocked by colchicine. Using thymocytes of mice the mitotic activity in short-time cultures was inhibited (50%) by the following concentrations of the substances under study (EC50-values in mol/l): tetraethylthiuramdisulfide; (1; disulfiram) 4.

[The exclusion of nonspecific effects in testing potential anti-inflammatory agents following intraperitoneal administration].

The intraperitoneal (i.p.) administration of potential antiphologistics of the group of DL-2-phenylglycinalkylester hydrochlorids caused beside the inhibition of the carrageenan rat paw oedema a high increase of the glucocorticoid-induced tyrosin transaminase activity (TTA) in the liver.

Effects of diethyl maleate on non-protein sulfhydryl content and cellular functions of mouse thymocytes in vitro.

The electrophilic compound diethyl maleate (DEM, 10(-5)-10(-3) M) decreased the content of non-protein sulfhydryl groups in mouse thymocytes in vitro and affected mitotic activity, spontaneous migration and viability of the cells. The mitotic activity of mouse thymocytes was most sensitive to DEM. It is probable that the decrease of the cell content of reduced glutathione (GSH) led to a failure of GSH-dependent cellular functions, such as mitosis and migration.

[Non-protein sulfhydryl groups and glutathione-S-transferases in acute toxic effects of Di-n-alkyltin dichlorides in mice and rats].

Toxic doses of di-n-butyl-tinn-dichloride (DBTC, 1.6 mmol/kg p.o.

Meso-2,3-dimercaptosuccinic acid increases the inhibition of glutathione S-transferase activity from rat liver cytosol supernatants by di-n-butyltin dichloride.

Di-n-butyltin dichloride (DBTC) and tricyclohexyltin chloride (TCHTC) inhibited the glutathione S-transferase activity of rat liver cytosolic supernatants towards 1-chloro-2,4-dinitrobenzene. Meso-2,3-dimercaptosuccinic acid increased inhibition of glutathione S-transferase by DBTC but lowered inhibition of the enzyme by TCHTC.

Ecto-ATPase activity in rat and mouse thymocytes and cytotoxic effects of various chemical substances in vitro.

Ecto-ATPase was present in thymocytes of mice and rats with higher activity in rat thymocytes. In rat but not in mouse thymocytes the ecto-ATPase was inhibited in vitro by HgCl2, tricyclohexyltin chloride, di-n-butyltin dichloride, DDT, gamma HCH and desipramine. The EC50 values ranged from 8 X 10(-7) mol/l for HgCl2 to 4 X 10(-4) mol/l for desipramine.