UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood.

Integrating nucleic acid extraction in amplification-based point-of-care diagnostics will be a significant feature for next-generation point-of-care virus detection devices. However, extracting DNA efficiently on a microfluidic chip poses many technological and commercialization challenges, including manual steps, multiple instruments, pretreatment processes, and the use of organic solvents (ethanol, IPA) that inhibit detection, which is not viable with routine testing such as viral load monitoring of transplant patients for post-operative care. This paper presents a microfluidic system capable of two-step DNA extraction from blood using a UV-assisted hyperbranched poly(β-amino ester) (HPAE)-modified silica membrane for cytomegalovirus (CMV) detection in a rapid and instrument-free manner without the presence of amplification inhibitors.

Permanent Hydrophobic Surface Treatment Combined with Solvent Vapor-Assisted Thermal Bonding for Mass Production of Cyclic Olefin Copolymer Microfluidic Chips.

A hydrophobic surface modification followed by solvent vapor-assisted thermal bonding was developed for the fabrication of cyclic olefin copolymer (COC) microfluidic chips. The modifier species 1,1,2,2-perfluorooctyl trichlorosilane (FOTS) was used to achieve the entrapment functionalization on the COC surface, and a hydrophobic surface was developed through the formation of a Si-O-Si crosslink network. The COC surface coated with 40 vol % cyclohexane, 59 vol % acetone, and 1 vol % FOTS by ultrasonic spray 10 and 20 times maintained its hydrophobicity with the water contact angle increasing from ∼86 to ∼115° after storage for 3 weeks.

Perspectives in translating microfluidic devices from laboratory prototyping into scale-up production.

Transforming lab research into a sustainable business is becoming a trend in the microfluidic field. However, there are various challenges during the translation process due to the gaps between academia and industry, especially from laboratory prototyping to industrial scale-up production, which is critical for potential commercialization. In this Perspective, based on our experience in collaboration with stakeholders, e.

A centrifugal microfluidic pressure regulator scheme for continuous concentration control in droplet-based microreactors.

Droplet microfluidics is an emerging tool in many biological and chemical application areas such as digital polymerase chain reaction (PCR) and in vitro diagnosis because of its extremely small sample volume and wide range of possibilities for on-demand adjustment of droplet properties. Although centrifugal microfluidics has been reported as a viable scheme for droplet generation, there is not much progress as far as droplet manipulation and droplet-based reactions are concerned. In this paper, we report a microfluidic pressure regulator scheme along with the use of microcapillaries for periodic droplet generation and the subsequent fusion.

Target trapping and in situ single-cell genetic marker detection with a focused optical beam.

Optical trapping of single particles or cells with the capability of in situ bio-sensing or genetic profiling opens the possibility of rapid screening of biological specimens. However, common optical tweezers suffer from the lack of long-range forces. Consequently, their application areas are predominantly limited to target manipulation instead of biological diagnostics.

Real-time multi-channel SPR sensing based on DMD-enabled angular interrogation.

This paper reports a digital micro-mirror device (DMD)-enabled real-time multi-channel biosensing system based on angular interrogation surface plasmon resonance (SPR). In the experiments, angular scanning is achieved by a DMD that facilitates SPR measurements using a single-point photodetector. In the four-channel measurement setup, real-time monitoring of bovine serum albumin (BSA) and anti-BSA binding interactions is performed at various concentration levels.

Thermal gradient induced tweezers for the manipulation of particles and cells.

Optical tweezers are a well-established tool for manipulating small objects. However, their integration with microfluidic devices often requires an objective lens. More importantly, trapping of non-transparent or optically sensitive targets is particularly challenging for optical tweezers.