EsigPBP3 Was the Important Pheromone-Binding Protein to Recognize Male Pheromones and Key Eucalyptus Volatiles.

Pheromone-binding proteins (PBPs) are specific odorant-binding proteins that can specifically recognize insect pheromones. Through transcriptional analysis of the antennae of adult , was discovered and identified, and was found to be highly expressed in the antennae of male moths. Based on the binding characteristics and ability of EsigPBP3, we can find the key ligands and binding site to consider as a target to control the key wood bore In this study, the fluorescence competitive binding assays (FCBA) showed that EsigPBP3 had a high binding affinity for seven key eucalyptus volatiles.

A Study of Adult Olfactory Proteins of Primitive Ghost Moth, (Lepidoptera, Hepialidae).

is a prominent wood-boring insect species in eucalyptus plantations in Guangxi, China, causing significant ecological and economic damage. A novel approach to controlling the challenging wood-boring pest involves disrupting the olfactory communication between insects and the volatile compounds emitted by plants. To identify the olfactory proteins contributing to host selection based on 11 GC-EAD-active volatiles from eucalyptus leaves and to discover the highly expressed olfactory proteins, we conducted a study on the antennal transcriptomes of adult and screened key olfactory proteins in the antennae.

: The Key Protein Binding Alpha-Phellandrene in Larvae.

larvae show olfactory recognition towards volatiles of eucalyptus trunks and humus soils. Further, was identified through larval head transcriptome and speculated as the main odorant-binding proteins in larvae. In this study, the highest expression of was only expressed in the heads of 3rd instar larvae of , compared with the thorax and abdomen; this was consistent with the phenomenon of habitat transfer of 3rd instar larvae, indicating that was a key OBP gene in larvae.