Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach.

Background: Histone ubiquitination modification is emerging as a critical epigenetic mechanism involved in a range of biological processes. In vitro reconstitution of ubiquitinated nucleosomes is pivotal for elucidating the influence of histone ubiquitination on chromatin dynamics.

Results: In this study, we introduce a Non-Denatured Histone Octamer Ubiquitylation (NDHOU) approach for generating ubiquitin or ubiquitin-like modified histone octamers.

Research Status of the Orphan G Protein Coupled Receptor 158 and Future Perspectives.

G-protein-coupled receptors (GPCRs) remain one of the most successful targets for therapeutic drugs approved by the US Food and Drug Administration (FDA). Many novel orphan GPCRs have been identified by human genome sequencing and considered as putative targets for refractory diseases. Of note, a series of studies have been carried out involving GPCR 158 (or GPR158) since its identification in 2005, predominantly focusing on the characterization of its roles in the progression of cancer and mental illness.

A photoactivatable antibody-Chlorin e6 conjugate enabling singlet oxygen production for tumor-targeting photodynamic therapy.

Photodynamic therapy is a new technology for disease diagnosis and treatment in modern medical clinics. The main advantages of photodynamic therapy are low toxicity and side effects, a wide range of applications, no drug resistance, and no obvious trauma in the treatment process. However, to achieve effective photodynamic therapy, new photosensitizer carriers need to be constructed, which can selectively deliver photosensitizers into tumor tissues.

Structural basis for ligand recognition of the human thromboxane A receptor.

Stimulated by thromboxane A, an endogenous arachidonic acid metabolite, the thromboxane A receptor (TP) plays a pivotal role in cardiovascular homeostasis, and thus is considered as an important drug target for cardiovascular disease. Here, we report crystal structures of the human TP bound to two nonprostanoid antagonists, ramatroban and daltroban, at 2.5 Å and 3.

Relationship between Contrast-Enhanced CT and Clinicopathological Characteristics and Prognosis of Non-Small Cell Lung Cancer.

Background: This study investigated the relationship between contrast-enhanced computed tomography (CECT) and clinicopathological characteristics and prognosis of non-small cell lung cancer (NSCLC).

Methods: A total of 198 NSCLC patients admitted to Enze Hospital from February 2009 to July 2012 underwent pre-surgical CECT to investigate parameters such as tumor size, CECT enhancement, lymph node enlargement, and lymph node size. Chi-square and log-rank tests were used to analyze associations between CECT parameters and pathological features as well as correlations of CECT parameters with prognosis.