Publications by authors named "Heng-yi Guo"
Article Synopsis
- The study aimed to understand how alpha-zearalanol (alpha-ZAL) affects the production of reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs) stimulated by tumor necrosis factor-alpha (TNF-alpha).
- Alpha-ZAL reduced ROS levels and the expression of the protein p47(phox) associated with ROS production in cells previously treated with TNF-alpha, indicating a protective effect.
- The results suggest that alpha-ZAL can inhibit key signaling pathways that lead to ROS generation and the activation of transcription factors involved in inflammation, highlighting its potential therapeutic role.
Objective: To investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs).
Methods: The mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of plasmid NF-kappaB-LUC.
[Identification of a novel splice mutation of low density lipoprotein receptor gene in a Chinese family with familial hypercholesterolemia].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
February 2004
Objective: To identify the mutation of low density lipoprotein receptor(LDLR) gene in a large Chinese family with familial hypercholesterolemia(F H) and make a discussion on the pathogenesis of FH at the molecular level.
Methods: Investigations were made on a patient with the clinical phenotype of homozygous FH and his parents for mutations of promoter and all 18 exons of LDLR gene. Screening was carried out using Touch down PCR and a g arose gel electrophoresis, combined with DNA sequence analysis.