The location of invasion-related protein MIC3 of Toxoplasma gondii and protective effect of its DNA vaccine in mice.

The micronemal protein MIC3 of Toxoplasma gondii plays a predominant role in the early phase of the invasion process. In this research, the expression and location of protein MIC3 and fusion protein EGFP-MIC3 were observed in host cells and in T. gondii respectively.

[Expressions of survivin and nm23 and the relationship among the expressions and the axillary lymph node metastasis in breast cancer].

Objective: To investigate the expressions of Survivin protein and nm23 protein and the relationship among the expressions and axillary lymph node metastasis in breast cancer.

Methods: The expression of Survivin and nm23 in 80 cases of breast cancer tissues were detected by immunohistochemistry SP method, and their correlation with axillary lymph node metastasis and 5-year disease free survival (DFS) were analysed.

Results: Survivin protein positive expression rate was 68.

[Analysis on morbidity and chemotherapy effects of Schistosoma japonicum infection in fishermen on Dongting Lake].

Objective: To clarify and evaluate the morbidity of schistosome infection and the effectiveness of chemotherapy among fishermen on East Dongting Lake.

Methods: Information on water-contact, history of infection and of praziquantel (PZQ) treatment among fishermen was collected. Kato-Katz method and miracidium hatching test were applied to detect the pathogens in stool specimen.

[Construction and sequencing of recombinant plasmid pcDNA3/GRA1 from Toxoplasma gondii].

Objective: To construct a mammalian expression plasmid pcDNA3/GRA1 to express dense granules antigen-1 (GRA1) of Toxoplasma gondii, and to lay a foundation for further studying the protective immunity of pcDNA3/GRA1 as a DNA vaccine.

Methods: The GRA1 opening reading frame (ORF) was amplified with two specific primers. The ORF and plasmid pcDNA3 were digested with EcoR I and Xho I respectively and the ORF was ligated into the pcDNA3 at polylinker.

[Immunological screening of Toxoplasma tachyzoite cDNA expression libraries with serum from infected rats].

Objective: To screen and identify the potential candidates for the development of toxoplasmosis vaccine.

Methods: Rats were infected with Toxoplasma gondii (T. gondii) RH strain and their sera were used as a probe to screen T.

[Improvement in the dot immunogold filtration assay].

Objective: To improve the filtrating unit of the dot immunogold filtration assay (DIGFA) to reduce its cost and make it suitable for fieldwork.

Methods: A thick round filtrating paper disc was made to replace the plastic filtrating box, and their results were compared.

Results: The size of the self-made filtrating paper disc was smaller and the cost was lower.