Hydraulic conductivity and microscopic properties of polyanionic cellulose and microscale zero-valent iron amended sand/bentonite backfills exposed to dichloromethane solution.

Leachate comprising organic contaminants such as dichloromethane is frequently discharged into groundwater at contaminated sites and unlined landfills. Soil-bentonite backfills in vertical cutoff walls are extensively employed to contain the flow of contaminated groundwater, thereby safeguarding the downstream groundwater environmental quality and ecosystem. This study presented a comprehensive evaluation of effects of dichloromethane-impacted groundwater on hydraulic conductivity and microscopic characteristics of soil-bentonite backfills amended with polymer namely polyanionic cellulose and microscale zero-valent iron.

Transit amplifying cells coordinate mouse incisor mesenchymal stem cell activation.

Stem cells (SCs) receive inductive cues from the surrounding microenvironment and cells. Limited molecular evidence has connected tissue-specific mesenchymal stem cells (MSCs) with mesenchymal transit amplifying cells (MTACs). Using mouse incisor as the model, we discover a population of MSCs neibouring to the MTACs and epithelial SCs.

Prominin-1 controls stem cell activation by orchestrating ciliary dynamics.

Proper temporal and spatial activation of stem cells relies on highly coordinated cell signaling. The primary cilium is the sensory organelle that is responsible for transmitting extracellular signals into a cell. Primary cilium size, architecture, and assembly-disassembly dynamics are under rigid cell cycle-dependent control.

[Biological effect of inhibition of Notch signaling pathway on human dental pulp cells].

Objective: To investigate the effect of Notch signaling pathway on human dental pulp cells.

Methods: The γ-secretase inhibitor N-[N-(3, 5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester(DAPT) was applied to inhibit the Notch signaling pathway of human dental pulp cells. The solvent dimethly sulfoxide (DMSO) served as the negative control.

[Notch activation delayed ageing of human dental pulp cells].

Objective: To establish model of dental pulp cells with activated Notch signaling pathway, and investigate the effect of activating Notch signaling pathway on senescence of human dental pulp cells in vitro.

Methods: Human dental pulp cells were isolated, cultured as usual, and used from the 4(th) passage. The cells were divided into the activated group and the negative control group.

[Effects of DAPT on proliferation of human dental pulp cells and Notch signaling pathway].

Objective: To explore whether the γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) could inhibit Notch signaling pathway in human dental pulp cells, and its effects on the proliferation ability of the cells.

Methods: Human dental pulp cells were primarily cultured from healthy premolars or wisdom teeth extracted intactly. The γ-secretase inhibitor DAPT (5 μmol/L) was added to the culture medium from passage 4 to the end.

Involvement of Notch signalling pathway in senescence of human dental pulp cells.

Objective: To evaluate whether the Notch signalling pathway is involved in the senescence of human dental pulp cells.

Methods: Human dental pulp cells were isolated and cultured. The Notch signalling pathway was blocked by adding DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester, γ-secretase inhibitor, 5 μmol/L) into the culture medium.

[Observation of multi-directional differentiation capability of dental papilla cells].

Objective: To study the types of progenitors in dental papilla cells(DPCs) and their differentiation characteristics.

Methods: DPCs of the mandibular first molars of four-day post-natal SD rats were isolated and cultured. The expression of osteocalcin (OCN) and dentin sialoprotein (DSP) were detected using DAB kit.

[Genetic diversity of F-ATPase subunits gene uncEBF amplified from Streptococcus mutans clinical isolates].

Objective: The purpose of this research was to study the genetic diversity of F-ATPase subunit gene uncEBF derived from Streptococcus mutans (S. mutans) clinical isolates, furthermore to investigate the relationship between the genetic diversity of F-ATPase and S. mutans aciduric ability.

Differentiation of dental pulp stem cells into regular-shaped dentin-pulp complex induced by tooth germ cell conditioned medium.

Investigations of the odontoblast phenotype are hindered by obstacles such as the limited number of odontoblasts within the dental pulp and the difficulty in purification of these cells. Therefore, it is necessary to develop a cell culture system in which the local environment is inductive and can promote dental pulp stem cells (DPSCs) to differentiate into odontoblast lineage. In this study, we investigated the effect of conditioned medium from developing tooth germ cells (TGCs) on the differentiation and dentinogenesis of DPSCs both in vitro and in vivo.

[Genetic diversity and mRNA expression of F-ATPase subunit uncG gene of streptococcus mutans clinical isolates].

Objective: To study the genetic diversity and the gene expression of membrane-bound proton-translocating ATPase (F-ATPase) subunit gene uncG derived from Streptococcus mutans (S. mutans) clinical isolates.

Methods: 38 S.

[A study of genetic diversity in lactate dehydrogenase of Streptococcus mutans from clinical isolates].

Objectives: Lactate dehydrogenase (LDH) is shown to be an important virulence factor resulting in acid production of Streptococcus mutans (S. mutans), on which the cariogenic potential of S. mutans depends.

[Homology analysis of the extended-V region of the surface proteins in different serotype Streptococcus mutans].

Objective: To analysis the homology among the extended-V region of the surface proteins in different serotype Streptococcus mutans (c, f, d, g) and to find out it's significance in anti-caries vaccine.

Methods: The DNA of the bacteria (standarded serotype c, d, f, g and partial serotype c clinicals) was extracted and the extended-V region (SrV+, 1 384-2 514 bp) was amplified using polymerase chain reaction (PCR). Then the products were assessed using restriction fragment length polymorphism (RFLP) by endonuclease Dde I.

[Study on the genetic diversity within the extended-V region of the surface protein of Streptococcus mutans].

Purpose: The purpose of this study was to investigate the genicity of the extended- variable region (V+) of surface proteins of Streptococcus mutans (S. mutans, serotype c,f) and provide some genetic informations on the molecular structure of S. mutans surface protein.

Cell pellets from dental papillae can reexhibit dental morphogenesis and dentinogenesis.

We isolated dental papilla mesenchymal cells (DPMCs) from different rat incisor germs at the late bell stage and incubated them as cell pellets in polypropylene tubes. In vitro pellet culture of DPMCs presented several crucial characteristics of odontoblasts, as indicated by accelerated mineralization, positive immunostaining for dentin sialophosphoprotein and dentin matrix protein 1, and expression of dentin sialophosphoprotein mRNA. The allotransplantation of these pellets into renal capsules was also performed.

[Genetic diversity of F-ATPase subunits gene uncA amplified from Streptococcus mutans clinical isolates].

Objective: To study the genetic diversity of F-ATPase alpha subunit gene uncA derived from Streptococcus mutans (S. mutans) clinical isolates and to investigate the relationship between the genetic diversity of acidurance factor and S. mutans aciduric ability, also and the cariogenicity.

[Study on lactate dehydrogenase activity of Streptococcus mutans isolates derived from caries-active and caries-free individuals].

Objective: To preliminarily investigate the relationship between the Lactate dehydrogenase (LDH) activity of Streptococcus mutans (S. mutans) and dental caries initiation.

Methods: 100 S.

[Relationship of the genetic diversity within the V-region, P-region and C-terminus of surface protein of Streptococcus mutans to dental caries].

Objective: To gain an understanding of the relationship between the genetic diversity within the V-region, P-region, C-terminus of surface protein of Streptococcus mutans (serotype c) and their adherent abilities.

Methods: The clinical isolates of S. mutans (serotype c) in two groups with different adherent abilities (cpm>2000, cpm<1000) had been prepared in our experimental laboratory.

[Detection of the transmitted strains and non-transmitted strains of Mutans streptococci by AP-PCR].

Objective: Dental caries is a transmissible infectious disease in which S. mutans plays the major role. The purpose of this study was to detect the S.

[A study on screening effective immunization route of anticaries DNA vaccine pcDNA3-gtfB].

Objective: Glucosyltransferase-B (GTF-B) of Streptococcus mutans has been implicated as a principal virulent factor in the development of dental caries. The objective was to use recombined plasmid pcDNA-gtfB expressing multiple antigen of glucosyltransferase-B as gene vaccine to immunize rats through different route, and to investigate the immunization effects of immunization routes.

Methods: A total of 18 Wistar rats were divided into 3 groups, including the quadriceps injection group, the intransal irrigation group and the submandibular gland-targeted injection group.