The effect of integrated health management model on the satisfaction among Chinese elderly.

The study objective was to investigate the effect of integrated health management model on the satisfaction among Chinese elderly. Using a randomized controlled trial, 600 people who are 60 and over have signed the informed consent in China were randomly assigned into management and control group. The management group received care integrated individual health management with community health management model in the follow-up 6 months.

The effects of qindan-capsule-containing serum on the TGF-β1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts.

Context: Qindan capsule (QC), a compound used in traditional Chinese medicine, has been used as an anti-hypertensive agent in clinical settings for years. Our previous studies have shown that QC can improve the morphological index of the artery, down-regulate the collagen volume fraction in the media and inhibit the transformation of smooth muscle cells. However, the detailed mechanisms underlying its effects require further investigation, which might provide more scientific evidence for the clinical treatment of hypertensive vascular remodeling (VR).

Modeling nutrient release in the Tai Lake basin of China: source identification and policy implications.

Because nutrient enrichment has become increasingly severe in the Tai Lake Basin of China, identifying sources and loads is crucial for watershed nutrient management. This paper develops an empirical framework to estimate nutrient release from five major sectors, which requires fewer input parameters and produces acceptable accuracy. Sectors included are industrial manufacturing, livestock breeding (industrial and family scale), crop agriculture, household consumption (urban and rural), and atmospheric deposition.

Enhancement of the immunogenicity and protective efficacy of a mucosal influenza subunit vaccine by the saponin adjuvant GPI-0100.

Identification of safe and effective adjuvants remains an urgent need for the development of inactivated influenza vaccines for mucosal administration. Here, we used a murine challenge model to evaluate the adjuvant activity of GPI-0100, a saponin-derived adjuvant, on influenza subunit vaccine administered via the intranasal or the intrapulmonary route. Balb/c mice were immunized with 1 µg A/PR/8 (H1N1) subunit antigen alone or in combination with varying doses of GPI-0100.

[Dermal hypoesthesia after total knee arthroplasty].

Objective: To observe the incidence of skin sensory loss after total knee arthroplasty (TKA) and its natural history over time, and to identify the relationship between numbness area and incision length, tourniquet time, age and gender.

Methods: In the study, 132 patients (20 males and 112 females, with an average age of 69.75 years old, 135 cases of TKA) who underwent primary TKA with midline incisions were chosen and grouped chronologically (4 years, 3 years, 2 years, 1 year, 6 months, 1 month) to the investigation time point from Peking University First Hospital.

QXAFS system of the BL14W1 XAFS beamline at the Shanghai Synchrotron Radiation Facility.

The quick-scanning XAFS (QXAFS) method is achieved at the BL14W1 XAFS beamline at the Shanghai Synchrotron Radiation Facility based on the EPICS and LabVIEW systems. This is realised by the unprecedented use of LabVIEW's data logging and supervisory control module for communication with EPICS in synchrotron radiation facilities. A fine QXAFS spectrum with an energy range of 1.

A mirage study of CdSe colloidal quantum dot films, Urbach tail, and surface states.

Thermal deflection spectroscopy allows to measure very small absorption and uncovers absorption tails extending well below the bulk bandgap energy for CdSe quantum dots films after ligand exchange by sulfide. In this monodispersed system, the redshift, the broadening, and the absorption tails cannot be solely attributed to electronic coupling between the dots. Instead, mixing of hole states from the quantum dot and surface is proposed to dominate the changes of the interband spectra at the absorption edge.

MAIGO2 is involved in abscisic acid-mediated response to abiotic stresses and Golgi-to-ER retrograde transport.

The central role of multisubunit tethering complexes in intracellular trafficking has been established in yeast and mammalian systems. However, little is known about their roles in the stress responses and the early secretory pathway in Arabidopsis. In this study, Maigo2 (MAG2), which is equivalent to the yeast Tip20p and mammalian Rad50-interacting protein, is found to be required for the responses to salt stress, osmotic stress and abscisic acid in seed germination and vegetative growth, and MAG2-like (MAG2L) is partially redundant with MAG2 in response to environmental stresses.

Construction and secretory expression of beta-galactosidase gene from Lactobacillus bulgaricus in Lactococcus lactis.

Objective: This study is to examine the secretion effects of beta-galactosidase in Lactococcus lactis.

Methods: The usp45 and beta-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant plasmid pMG36e-usp-lacZ. This recombinant plasmid was transformed into both Escherichia coli DH5alpha and L.

A novel molecular probe sensing polynuclear hydrolyzed aluminum by chelation-enhanced fluorescence.

A novel molecular probe (P2) sensing polynuclear hydrolyzed aluminum by chelation-enhanced fluorescence was described, which was prepared from 1,1'-(1,4-phenylene)bis(7-methyl-1,3,5-octanetrione) and 2-aminomethylpyridine. P2 showed a strong fluorescence response to Al(III) when excited by the optimized wavelength of 400 nm and the λ(max,em) at 505 nm indicated a hypsochromic shift of 11 nm to that of free P2, but Cu(II) was observed to quench the fluorescence intensity to nearly zero. The P2-Al(III) complex indicated the best fluorescence response at a pH near 7 and there might be a relevance to the amphoteric property of the aluminum hydroxide.

Highly sensitive method for assay of drug-induced apoptosis using fluorescence correlation spectroscopy.

Apoptosis plays a crucial role in many biological processes and pathogenesis of various malignancies and diseases of the immune system. In this paper, we described a novel method for sensitive detection of drug-induced apoptosis by using fluorescence correlation spectroscopy (FCS). The principle of this method is based on the assay of DNA fragmentation in the process of the drug-induced apoptosis.

Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.

Background: Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum.

Methods And Main Results: Synaptic responses of MLIs and Purkinje cells (PCs), evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques.

Azepine synthesis from alkyl azide and propargylic ester via gold catalysis.

An efficient new method was developed to synthesize multisubstituted 4,5-dihydro-1H-azepine derivatives through the gold-catalyzed reaction of two molecules of propargylic esters with one molecule of alkyl azide. It was proposed that vinyl gold carbenoid, in situ generated from propargylic ester through gold-catalyzed 1,2-rearrangement, was trapped by alkyl azide to give vinyl imine intermediate. These, in turn, could undergo a formal [4 + 3] cycloaddition with another molecule of vinyl gold carbenoid to afford the desired azepine product.

Spatially resolved scattering correlation spectroscopy using a total internal reflection configuration.

In the paper, we present a novel single particle method, named spatially resolved scattering correlation spectroscopy (SRSCS), based on a total internal reflection (TIR) configuration and strong resonance light scattering (RLS) of silver nanoparticles (AgNPs). The principle of SRSCS is similar to fluorescence correlation spectroscopy (FCS), and it is based on measuring the RLS fluctuations in a small volume due to Brownian motion of single nanoparticles. We first established a highly sensitive SRSCS system.

Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana.

Previous studies have shown that the late embryogenesis abundant (LEA) group 3 proteins significantly respond to changes in environmental conditions. However, reports that demonstrate their biological role, especially in Arabidopsis, are notably limited. This study examines the functional roles of the Arabidopsis LEA group 3 proteins AtLEA3-3 and AtLEA3-4 in abiotic stress and ABA treatments.

Colloidal stability of gold nanoparticles modified with thiol compounds: bioconjugation and application in cancer cell imaging.

Gold nanoparticles (GNPs) are attractive alternative optical probes and good biocompatible materials due to their special physical and chemical properties. However, GNPs have a tendency to aggregate particularly in the presence of high salts and certain biological molecules such as nucleic acids and proteins. How to improve the stability of GNPs and their bioconjugates in aqueous solution is a critical issue in bioapplications.

In vitro and in vivo inhibitory effect of the combination of Wenxia Changfu formula [see text] with cisplatin in non-small cell lung cancer.

Objective: To observe the effect of the combination of Wenxia Changfu Formula ([see text], WCF) with cisplatin (CDDP) on inhibiting non-small cell lung cancer (NSCLC) in vitro and In Vivo and explore its mechanism from its effect on cell cycle.

Methods: In vitro, WCF-containing serum was prepared and the rhubarb b1, emodin, and aconitine were detected qualitatively by high-performance liquid chromatogram (HPLC). A549 cell lines were treated with blank control (dimethyl sulfoxide), normal serum, normal serum with CDDP (1.

UXT-V1 facilitates the formation of MAVS antiviral signalosome on mitochondria.

Virus infection induces the MAVS-TNFR-associated factor (TRAF) 3 signaling axis on mitochondria. It remains to elucidate the corresponding regulatory processes. In this study, we identify UXT-V1 as a novel TRAF3-binding protein.

Effect of Da-Cheng-Qi-Tang on gastrointestinal motility in patients undergoing laparotomy.

Background/aims: The aim of this study was to investigate the effect of Da-Cheng-Qi-Tang (DCQT) on the gastrointestinal motility after abdominal surgery in humans.

Methodology: Thirty-three patients with abdominal surgery and 36 patients with cholecystectomy were divided into DCQT and control groups at random. Electrogastrography (EGG) and antroduodenojejunal manometry were performed and the level of plasma ghrelin were measured by ELISA.

Small heat shock protein LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily.

Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers at various stages of development by the pollen mother cells.

[Phosphorus flow analysis of civil food production and consumption system].

With the method of substance flow analysis (SFA), the study provided an analytical framework for phosphorus cycle on civil food production and consumption system that was demonstrated by four life stages-food production, food processing, consumption and waste handling. Furthermore, a case study was carried out in Hefei City, for the year 2008. The SFA results show that the total demand of phosphorus reaches 5 475.

UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation.

Proteins that directly regulate tumor necrosis factor (TNF) signaling have critical roles in determining cell death and survival. Previously we characterized ubiquitously expressed transcript (UXT)-V2 as a novel transcriptional cofactor to regulate nuclear factor-κB in the nucleus. Here we report that another splicing isoform of UXT, UXT-V1, localizes in cytoplasm and regulates TNF-induced apoptosis.

[Cloning and expression of a single human immunoglobulin heavy-chain variable domain with vascular endothelial growth factor binding activity].

In the application of therapeutic antibodies, large molecular weight of antibodies is always a problem that prevents them from penetrating into tissues or binding to antigenic determinants. To overcome this problem, we investigated the function of the heavy chain variable domain of a monoclonal anti-VEGF human IgM antibody derived from the Five-Feature Translocus Mice. We cloned the cDNA of the heavy chain variable domain, which was then inserted into pET28a vector and expressed in Escherichia coli.

REST regulates DYRK1A transcription in a negative feedback loop.

DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1A) has been shown to be involved in learning and memory impairments in Alzheimer disease and Down syndrome. As a homolog of Drosophila minibrain gene, DYRK1A also plays important roles in neurodevelopment; however, the function and regulatory mechanism of DYRK1A in neurodevelopment remain elusive. REST (RE1 silencing transcription factor) plays vital roles in neuronal differentiation.