Protein arrays from cDNA expression libraries.

This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the array for binding proteins. After producing the library and decollating the expression clones, one can pick thousands of expression clones with a laboratory robot and can deposit them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting material for the construction of a high-density protein array.

Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction.

The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.