Publications by authors named "Hendrickx A"

We studied nosocomial transmission of multidrug-resistant bla-containing Klebsiella pneumoniae ST147 in a Dutch pediatric oncology center. Whole-genome multilocus sequence typing revealed two genetic clusters consisting of 2 and 5 K. pneumoniae isolates, both from Ukrainian medical evacuees and Dutch patients.

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  • Multidrug-resistant organisms (MDRO) are a major public health concern, and understanding their genomic characteristics is vital for monitoring and controlling infections.
  • This study focuses on the use of long-read whole-genome sequencing (WGS) to analyze the molecular profiles of 356 MDRO isolates, including various bacteria such as Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA).
  • Results showed that long-read sequencing provides comparable molecular data to short-read sequencing, with high consistency in multi-locus sequence typing (wgMLST) profiles, though some differences were noted in Pseudomonas aeruginosa.
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  • A study in Romania revealed a growing prevalence of New Delhi metallo-beta-lactamase (NDM)-producing Enterobacterales in hospitals, with 355 cases reported from 2018 to 2022.
  • Analysis of 74 isolates over nearly two years identified four persistent multi-hospital clusters of these drug-resistant bacteria, demonstrating sustained transmission across multiple regions.
  • The findings highlight the urgent need for enhanced infection control measures and improved national surveillance for carbapenem-resistant pathogens in healthcare systems across the EU/EEA.
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  • * A total of 18 carbapenemase variants were identified, predominantly in ST131 clades A and C, with the most common variants being detected in 14 and 12 countries, respectively.
  • * The rise in isolates carrying these genes since 2021 highlights a significant public health threat posed by the high-risk ST131 lineage.
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Background: Vancomycin-resistant Enterococcus faecium (VRE) is an opportunistic pathogen, which can cause outbreaks in hospitals. In the Netherlands, several national guidelines and guidance documents on different aspects of VRE management are available. Most available guidelines are written towards the hospital setting and only few on long-term care facilities (LTCFs).

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  • A significant rise in multidrug-resistant microorganisms (MDRO) was noted following the transfer of Ukrainian patients to hospitals, with various resistant strains identified, including carbapenem-resistant bacteria.
  • Testing for antimicrobial susceptibility showed low efficacy rates for several last-resort antibiotics, highlighting severe resistance issues among these pathogens.
  • Factors influencing cefiderocol susceptibility results include testing methods and standards used, making understanding resistance patterns crucial for treating patients suspected of Gram-negative infections with recent hospitalizations in Ukraine.
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Objectives: We describe the clonal spread of New Delhi metallo-β-lactamase (NDM) 1-producing isolates belonging to the ST773 clone in Spain and the Netherlands, associated with the transfer of Ukrainian patients during the war.

Methods: Between March and December 2022, nine NDM-1-producing ST773 isolates were recovered from nine Ukrainian patients evacuated to two Spanish ( = 3) and five Dutch ( = 6) hospitals. Antimicrobial susceptibility testing was studied (Sensititre, Microscan, EUCAST-2023).

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Background: In the hospital environment, carbapenemase-producing Pseudomonas aeruginosa (CPPA) may lead to fatal patient infections. However, the transmission routes of CPPA often remain unknown. Therefore, this case study aimed to trace the origin of CPPA ST357, which caused a hospital-acquired pneumonia in a repatriated critically ill patient suffering from Guillain-Barré Syndrome in 2023.

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Background And Aims: Leaf traits are known to be strong predictors of plant performance and can be expected to (co)vary along environmental gradients. We investigated the variation, integration, environmental relationships and evolutionary history of leaf functional traits in the genus Coffea, typically a rainforest understorey shrub, across Africa. A better understanding of the adaptive processes involved in leaf trait evolution can inform the use and conservation of coffee genetic resources in a changing climate.

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Purpose: In critically ill adults, withholding parenteral nutrition until 1 week after intensive care admission (Late-PN) facilitated recovery as compared with early supplementation of insufficient enteral nutrition with parenteral nutrition (Early-PN). However, the impact on long-term mortality and functional outcome, in relation to the estimated nutritional risk, remains unclear.

Methods: In this prospective follow-up study of the multicenter EPaNIC randomized controlled trial, we investigated the impact of Late-PN on 2-year mortality (N = 4640) and physical functioning, assessed by the 36-Item Short Form Health Survey (SF-36; in 3292 survivors, responding 819 [738-1058] days post-randomization).

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Carbapenem-resistant (CRAb) is an important pathogen causing serious nosocomial infections. We describe an outbreak of CRAb in an intensive care unit in the Netherlands in 2021. During an outbreak of non-resistant , while infection control measures were in place, CRAb isolates carrying highly similar and encoding plasmids were isolated from three patients over a period of several months.

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In the Ross procedure, a patient's pulmonary valve is transplanted in the aortic position. Despite advantages of this surgery, reoperation is still needed in many cases due to excessive dilatation of the pulmonary autograft. To further understand the failure mechanisms, we propose a multiscale model predicting adaptive processes in the autograft at the cell and tissue scale.

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Biofilm formation is a critical step in the pathogenesis of difficult-to-treat Gram-positive bacterial infections. We identified that YajC, a conserved membrane protein in bacteria, plays a role in biofilm formation of the clinically relevant strain E1162. Deletion of conferred significantly impaired biofilm formation and was attenuated in a rat endocarditis model.

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BackgroundThe war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related to Ukrainian patients.AimTo investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing from Ukrainian patients among European countries.

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is an opportunistic pathogen able to colonize the intestines of hospitalized patients. This initial colonization is an important step in the downstream pathogenesis, which includes outgrowth of the intestinal microbiota and potential infection of the host. The impact of intestinal overgrowth on host-enterococcal interactions is not well understood.

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Article Synopsis
  • * Researchers analyzed 275 isolates from various countries using genomic techniques and found three main groups along with a proposed modified taxonomy for the genus.
  • * The findings suggest significant genetic diversity among Morganella spp and highlight the importance of monitoring these pathogens' resistance mechanisms for better treatment options.
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Background: Residents in psychiatry can be confronted with patient-related stressful experiences which can be impactful. Guidelines for dealing with these are lacking.

Aim: Questioning residents in psychiatry about patient-related stressful adversities, their impact and resources to contribute to the existing literature.

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Objectives: Recently, reports on antimicrobial-resistant Bacteroides and Prevotella isolates have increased in the Netherlands. This urged the need for a surveillance study on the antimicrobial susceptibility profile of Bacteroides, Phocaeicola, Parabacteroides and Prevotella isolates consecutively isolated from human clinical specimens at eight different Dutch laboratories.

Methods: Each laboratory collected 20-25 Bacteroides (including Phocaeicola and Parabacteroides) and 10-15 Prevotella isolates for 3 months.

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Objectives: Rupture and dissection are feared complications of ascending thoracic aortic aneurysms caused by mechanical failure of the wall. The current method of using the aortic diameter to predict the risk of wall failure and to determine the need for surgical resection lacks accuracy. Therefore, this study aims to identify reliable and clinically measurable predictors for aneurysm rupture or dissection by performing a personalized failure risk analysis, including clinical, geometrical, histologic, and mechanical data.

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During 2015-2022, a genetic cluster of OXA-48-producing uropathogenic Escherichia coli sequence type 127 spread throughout the Netherlands. The 20 isolates we investigated originated mainly from urine, belonged to Clermont phylotype B2, and carried 18 genes encoding putative uropathogenicity factors. The isolates were susceptible to first-choice antimicrobial drugs for urinary tract infections.

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Background: Although the Netherlands is a country with a low endemic level, methicillin-resistant Staphylococcus aureus (MRSA) poses a significant health care problem. Therefore, high coverage national MRSA surveillance has been in place since 1989. To monitor possible changes in the type-distribution and emergence of resistance and virulence, MRSA isolates are molecularly characterized.

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