Epidemiological characteristics and molecular identification of species among cases of imported malaria in Kuwait during the COVID-19 pandemic.

Cases of imported malaria are reported each year in several malaria non-endemic countries, including Kuwait. PCR testing is the ideal method for identification of the infecting spp. The present study documented the epidemiologic characteristics of molecularly confirmed cases of imported malaria in Kuwait during the first year of COVID-19 pandemic.

Key cytokines and hematological parameters in patients with uncomplicated falciparum malaria in Hodeidah, Yemen.

Immunity to malaria has a major role in controlling disease and pathogenesis with cytokine production being involved in almost every phase of the immune response. The present study aimed to assess hematological variables and to measure plasma levels of TNFα, IFNγ and IL10, their ratios, and their relation to parasitemia among patients with uncomplicated falciparum malaria in Hodeidah, Yemen. Forty patients with uncomplicated P.

Levels of Serum Ferritin and Hepcidin in Patients with Uncomplicated Falciparum Malaria in Hodeidah, Yemen: Considerations for Assessing Iron Status.

Understanding the key regulator of iron homeostasis is critical to the improvement of iron supplementation practices in malaria-endemic areas. This study aimed to determine iron indices and hepcidin (HEPC) level in patients infected with compared to apparently healthy, malaria-negative subjects in Hodeidah, Yemen. The study included 70 -infected and 20 malaria-negative adults.

Comparison of polymerase chain reaction-restriction fragment length polymorphism and assemblage-specific primers for characterization of in children.

Background: is a diarrheagenic eukaryotic parasite that consists of at least eight morphologically identical but genetically distinct genotypes. Human giardiasis is caused mainly by A and B assemblages.

Aim And Objectives: The study aimed to compare the performance of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and assemblage-specific primers in genotyping of .

Expression of microRNA-223 and microRNA-146b in serum and liver tissue of mice infected with Schistosoma mansoni.

MicroRNAs (miRNAs) play regulatory roles in several diseases. In schistosomiasis, the main pathological changes are caused by the granulomatous reaction induced by egg deposition. We aimed to study the changes in host miRNA-223 and miRNA-146b expression in relation to egg deposition and development of hepatic pathology in murine schistosomiasis mansoni.

Pre-and post-treatment evaluation of intestinal inflammation in and infected children: a community-based study.

and cause frequent infections in children in developing countries. However, the role of intestinal inflammation in their pathogenesis is still poorly understood. Faecal calprotectin (FC) level is used as an indicator of intestinal inflammation and neutrophil migration in the intestinal tract.

Detection and molecular identification of isolates from humans and cattle in northern Egypt.

is one of the less well-understood waterborne protozoa of medical and veterinary importance. The present study aimed to detect, identify, and subtype in cattle and humans in northern Egypt and to investigate the potential for zoonotic transmission. Fecal samples were collected from 136 humans (68 in contact with animals and 68 no animal contact) and 190 cattle from a rural area in Kafr El-Sheikh Province, Egypt.

Detection of assemblages A and B among children from three villages in the West Delta region, Egypt using assemblage specific primers.

is a common diarrheagenic parasite infecting children globally. It has been classified into eight morphologically identical but genetically distinct genotypes. Human infection is mainly associated with A and B assemblages with variable geographical distribution.

Performance of formalin ethyl acetate, percoll sedimentation and ELISA for the detection of infection in asymptomatic children.

This study aimed to assess the performance of formalin ethyl acetate (FEA)/modified Ziehl-Neelsen (MZN), and percoll technique/MZN for the diagnosis of cryptosporidiosis among asymptomatic children compared to ELISA coproantigen. The study was conducted on 100 children in a rural area in Kafr El-Sheikh governorate. Stool samples were collected and examined by the three techniques.

microRNA-155 Expression and Butyrylcholinesterase Activity in the Liver Tissue of Mice Infected with Toxoplasma gondii (Avirulent and Virulent Strains).

Purpose: Toxoplasma gondii is an apicomplexan parasite that exhibits distinct strain-related virulence patterns in mice. It can induce hepatic inflammation. The present study investigated MicroRNA-155 (miRNA-155) expression and butyrylcholinesterase (BChE) activity in the liver tissue of mice infected with virulent and avirulent strains of T.

Intestinal parasites among humans and their livestock animals in a rural community in Gharbia governorate, Egypt.

Our objective was to study the prevalence of intestinal parasitic infections among humans and their contact livestock animals in a rural community in Gharbia governorate, Egypt. The collected human and livestock animal fecal samples were examined by formol ether sedimentation technique and modified Ziehl-Neelsen stain. Human samples were further examined by the Kato-Katz technique.

in cattle slaughtered in North Egypt: Overestimation by the visual inspection method.

Background And Aim: The World Health Organization and the Food and Agriculture Organization list , a foodborne cestode, as the most widely distributed human tapeworm worldwide. The larval stage of , , causes cysticercosis in bovines and infects humans who eat raw or undercooked beef. The existing detection methods of in cattle depend on the visual inspection of meat.

Impact of Coinfection with Schistosoma mansoni on the Antibody Response to Helicobacter pylori.

Purpose: In many tropical areas, the coinfection of Schistosoma spp. and other pathogens is frequent. The impact of schistosomiasis on other infections has been demonstrated for several organisms.

Temporal expression of Toxoplasma stage-specific genes in brain tissue: coincidence with parasitological and histopathological findings in mice models.

In the intermediate hosts, tachyzoites of T. gondii predominate in the acute stage while bradyzoites persist inside tissue cysts with the potential for reactivation. The two stages exhibit different metabolic and antigenic characters.

Molecular Characterization of Giardia intestinalis Detected in Humans and Water Samples in Egypt.

Background: Giardia intestinalis is a common cause of gastrointestinal illness especially in children of developing countries. Giardia assemblages A and B are the major human infective genotypes.

Objective: The present study aimed to investigate the role of water supply in the epidemiology of giardiasis via genotyping G.

Irritable bowel syndrome in Egyptian patients: plausible risk factors and association with intestinal protozoa.

The pathogenesis of irritable bowel syndrome (IBS) is not yet clear. Our study suggested parasitic infection and other plausible risk factors among Egyptian IBS patients. We studied 40 IBS patients diagnosed according to Rome III criteria (Group I), 40 with other gastrointestinal symptoms (Group II) and 40 healthy controls (Group III).

Antiparasitic Activity of Mirazid, Myrrh Total Oil and Nitazoxanide Compared to Praziquantel on : Scanning Electron Microscopic Study.

Background: The development of new antischistosomal drug remains a pressing need and a vital challenge in front of many researchers through screening the natural or chemical substances for their potential activity as antischistosomal agents.

Methods: Five groups of -infected mice (n=10) were enrolled in this study, the G1 was infected non-treated control group. G2 was infected treated with praziquantel 500 mg/kg for 2 consecutive days.

Toxoplasma gondii seropositivity in renal patients: rate, pattern, predictors and related morbidity.

Infection is a significant cause of morbidity and mortality in patients with chronic kidney disease (CKD) due to their depressed immunity. T. gondii is a ubiquitous parasite that causes severe manifestations in immunocomprmised patients.

Understanding drug resistance in human intestinal protozoa.

Infections with intestinal protozoa continue to be a major health problem in many areas of the world. The widespread use of a limited number of therapeutic agents for their management and control raises concerns about development of drug resistance. Generally, the use of any antimicrobial agent should be accompanied by meticulous monitoring of its efficacy and measures to minimize resistance formation.

Genotype analysis of T. gondii strains associated with human infection in Egypt.

The distribution of Toxoplasma gondii genotypes varies from one geographic area to another. The present study aimed to determine T. gondii genotypes associated with human infection in Egypt.

Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin.

Microsporidia are emerging opportunistic parasites. Preservation of the biological properties of microsporidian spores is often required in research work. The present study compared two preservatives; zinc polyvinyl alcohol (zinc PVA) and potassium dichromate solutions for preservation of microsporidian spores separated from human faecal samples.

Cryptosporidiosis and other intestinal parasites in 3 Yemeni orphanages: prevalence, risk, and morbidity.

The epidemiological pattern of cryptosporidiosis and other intestinal parasites were studied among 3 orphanages in Sana'a City. A total of 292 orphans (225 males & 67 females), aged 4-20 years old were recruited. A questionnaire of demographic information, health status and other relevant information was filled out for each one.

Use of a non-mercury containing fixative for diagnosis of giardiasis.

In the present study, methanol was used instead of Schaudinn's fluid for preparation of permanently stained faecal smears. The efficacy of this method to detect Giardia lamblia trophozoites and cysts were compared to merthiolate iodine formalin (MIF) direct smear method and MIF concentration (MIFC) technique. Fresh faecal samples were collected from school students with gastrointestinal symptoms.

Levels of growth hormone, albumin and ferritin in human fascioliasis and schistosomiasis.

This study assessed levels of growth hormone, albumin and ferritin in human fascioliasis and schistosomiasis. Forty three patients and ten healthy parasite free subjects were included as controls. All were subjected to clinical, parasitological, and haematological examinations.

Alteration in circulating soluble Fas level in Toxoplasma seropositive pregnant women.

The role of sFas in human toxoplasmosis was designed, included 23 pregnant women serologically positive for anti-Toxoplasma antibodies, and ten sero-negative pregnant females constituted the control group. Antibody titer was assessed by indirect haemagglutination test. The presence of specific IgM antibodies was determined by comparing antibody titer before and after serum treatment with 2-mercapto-ethanol.