Genetic Mapping of , a New Lethal Allele of .

The mutation was generated in a Flp/FRT EMS screen for conditional mutations that cause growth and developmental defects in a genetic background that blocks apoptosis. The mutations were conditional, based on the allele being present on the starting chromosome, and blocking canonical apoptosis in a homozygous state. The mosaic eyes exhibit defects in eye development including patches of rough eye and irregular surface structure.

Genetic Mapping and Phenotypic Analysis of on Eye and Antennae Development in .

Genetic screens are valuable for identifying novel genes involved in the regulation of developmental processes. To identify genes associated with cell growth regulation in , a mutagenesis screen was performed. Undergraduate students participating in Fly-CURE phenotypically characterized the mutant which is associated with rough eyes and antennae overgrowth.

, an allele of , causes tissue overgrowth in the eye.

Mutant , generated via EMS mutagenesis in , was studied by undergraduate students participating in the Fly-CURE. After inducing genetically mosaic tissue in the adult eye, mutant tissue displays a robust increase in cell division and a rough appearance. Complementation mapping and sequence analysis identified a nonsense mutation in the gene , which we named ( ) due to observed increases in red-pigmented mutant tissue compared to controls.

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis.

The basement membrane (BM) - a specialized sheet of extracellular matrix present at the basal side of epithelial cells - is critical for the establishment and maintenance of epithelial tissue morphology and organ morphogenesis. Moreover, the BM is essential for tissue modeling, serving as a signaling platform, and providing external forces to shape tissues and organs. Despite the many important roles that the BM plays during normal development and pathological conditions, the biological pathways controlling the intracellular trafficking of BM-containing vesicles and how basal secretion leads to the polarized deposition of BM proteins are poorly understood.

Genetic mapping of , a pupal lethal mutation in .

An EMS mutagenesis screen was conducted in to identify growth control mutants. The multi-institution Fly-CURE consortium phenotypically characterized the mutant using the system which displayed a mutant lethal phenotype with reduced head development, and darkened ocular tissue. Complementation mapping was conducted to identify the affected gene.