Which specimens from a museum collection will yield DNA barcodes? A time series study of spiders in alcohol.

We report initial results from an ongoing effort to build a library of DNA barcode sequences for Dutch spiders and investigate the utility of museum collections as a source of specimens for barcoding spiders. Source material for the library comes from a combination of specimens freshly collected in the field specifically for this project and museum specimens collected in the past. For the museum specimens, we focus on 31 species that have been frequently collected over the past several decades.

Spider hosts (Arachnida, Araneae) and wasp parasitoids (Insecta, Hymenoptera, Ichneumonidae, Ephialtini) matched using DNA barcodes.

The study of parasitoids and their hosts suffers from a lack of reliable taxonomic data. We use a combination of morphological characters and DNA sequences to produce taxonomic determinations that can be verified with reference to specimens in an accessible collection and DNA barcode sequences posted to the Barcode of Life database (BOLD). We demonstrate that DNA can be successfully extracted from consumed host spiders and the shed pupal case of a wasp using non-destructive methods.

Pharmacodynamics and pharmacokinetics of intravesical mitomycin C upon different dwelling times.

The schedule in dosing of intravesical chemotherapy has thus far received little attention. Correlation of optimal contact time with bladder toxicity, as well as maximal chemotherapeutic effect for one of the drugs of first choice for intravesical instillation, i.e.

Urological complications following aorto-iliac reconstructive surgery.

The incidence of urological complications after aorto-iliac graft surgery was prospectively studied in 49 patients and retrospectively in 35 other patients operated on more than five years before. Follow-up of all 49 patients was one year. In one patient a mild asymptomatic hydronephrosis was discovered after four months.

Recurrence of superficial bladder carcinoma after intravesical instillation of mitomycin-C. Comparison of exposure times.

A total of 70 patients were treated with intravesical instillations of Mitomycin-C (MMC) following transurethral resection for superficial bladder carcinoma. In this randomised prospective study the influence of 2 exposure times (0.5 and 1 h) was studied in relation to tumour recurrence, recurrence interval, tumour progression and toxicity.

Behavior and resorption of mitomycin C following multiple intravesical administrations.

Although several pharmacological data of intravesical mitomycin C (MMC) are available now, data about resorption following subsequent intravesical instillations with different instillation times are lacking. We have analyzed MMC concentrations in blood plasma and urine following eight subsequent instillations, with 0.5- and 1.

Complications following vasectomy. A comparison between Mersilene and autosuture surgiclips.

Comparison of the use of autosuture surgiclips and Mersilene for occlusion of the vas deferens showed a significantly lower number of complications, such as local infections, granuloma and fistula, within a month following vasectomy, when autosuture surgiclips were used (0.005 less than p less than 0.01).

Mitomycin C resorption following repeated intravesical instillations using different instillation times.

In a prospective and randomized trial, 31 patients with superficial and/or CIS transitional cell carcinoma were treated by complete transurethral resection of all visible tumours, followed by instillation of 40 mg mitomycin C (MMC). The instillation time was 0.5 and 1.

Are biopsies from the prostatic urethra useful in patients with superficial bladder carcinoma?

Fifty male patients entered a Mitomycin C trial for superficial bladder cancer and biopsies in the prostatic urethra were taken with a cold cup biopsy forceps. In one patient this procedure failed. In 21 of 49 patients no abnormalities were detected but in the remaining 28 patients (57.

Treatment of urothelial carcinoma of the upper urinary tract following prostatocystectomy with mitomycin C instillation in the ileal loop.

We report a case of urothelial carcinoma of the upper urinary tract, which developed after prostatocystectomy. The patient was treated with instillations of 40 mg. mitomycin C in 40 ml.

A secondary vaginal stone. A case report.

A case is presented of a large calculus that was formed in the vagina of a 44-year-old woman within less than 4 months. Urinary salts from a vesico-vaginal fistula deposited around an inadvertently unremoved medical gauze.

Signet-ring cell carcinoma of the bladder and the prostate. Report of 4 cases.

During a 9-year period in our clinic 4 patients were seen with signet-ring cell carcinoma. 2 patients had primary signet-ring cell carcinoma of the bladder, 1 of the prostate. In the 4th case the tumor was located near the penoscrotal angle of the urethra.

The renal and perirenal abscess: the role of ultrasound and computed tomography in its diagnosis, localization and treatment.

Perirenal abscesses are difficult to diagnose by clinical examination and traditional radiographic techniques. Four cases are reported in which ultrasound and computed tomography were very helpful in this respect. The role in the diagnosis and management of such abscesses with these two new technics of renal and perirenal imaging is discussed.

[A 2d malignancy of the kidney in a patient with Hodgkin disease following full remission].

A second case of a secondary kidney tumour following radio- and chemotherapy of Hodgkin's disease is presented. The radiological findings with a sarcomatoid hypernephroma are described.

Ureter replacement by collagen and seromuscular parts of the large bowel in dogs.

The use of stemmed patches and tubes from the seromuscular layer of the colon wall to replace part of the ureter has proven unreliable because of complications such as urinary leakage and invagination, fibrosis, bone formation, shrinkage and disappearance of the intestinal wall due to ischemia and necrosis, causing hydronephrosis and pyelonephritis. The use of tubes and patches of tanned and untanned collagen, implanted in order to study the ingrowth of urothelium and possibly muscle cells, resulted in fibrosis, bone formation, rejection of the material and, in the case of the tubes, complete obstruction with hydronephrosis and destruction of the kidney.