Detection of single-molecule H2O2 signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes.

An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H2O2) as beneficial messengers in redox signalling pathways. The nature of H2O2 signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and temporally, at low concentrations. Here, we develop an array of fluorescent single-walled carbon nanotubes that can selectively record, in real time, the discrete, stochastic quenching events that occur as H2O2 molecules are emitted from individual human epidermal carcinoma cells stimulated by epidermal growth factor.

(+)-{1,2-Bis[(2R,5R)-2,5-diethyl-phospho-lan-1-yl]ethane-κP,P'}(η-cyclo-octa-1,5-diene)rhodium(I) tetra-fluoridoborate.

The title compound, [Rh(C(8)H(12))(C(18)H(36)P(2))]BF(4), exhibits a rhodium(I) complex cation with a bidentate bis-phosphine ligand and a bidentate η(2),η(2)-coordinated cyclo-octa-1,5-diene ligand. The ligands form a slightly distorted square-planar coordination environment for the Rh(I) atom. An intra-molecular P-Rh-P bite angle of 83.

(+)-{1,2-Bis[(2R,5R)-2,5-dimethyl-phospho-lan-1-yl]ethane-κP,P'}(η-cyclo-octa-1,5-diene)rhodium(I) tetra-fluorido-borate.

The title compound, [Rh(C(8)H(12))(C(14)H(28)P(2))]BF(4), exhibits a rhodium(I) complex cation with a bidentate bis-phosphine ligand and a bidentate η(2),η(2)-coordinated cyclo-octa-1,5-diene. Together the ligands create a slightly distorted square-planar cordination environment for the Rh(I) atom. There are three mol-ecules in the asymmetric unit and intra-molecular P-Rh-P bite angles of 82.

The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection.

A major challenge in the synthesis of nanotube or nanowire sensors is to impart selective analyte binding through means other than covalent linkages, which compromise electronic and optical properties. We synthesized a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNTs) that imparts rapid and selective fluorescence detection of nitric oxide (NO), a messenger for biological signalling. The near-infrared (nIR) fluorescence of SWNT(DAP-dex) is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species.

Low probability of Significant Intra-Abdominal Injury in Stable Patients with Abdominal ''Seat Belt Sign''.

Study Objective: Our aim was to determine the diagnostic significance of the association between the abdominal skin bruise from a seat belt and the presence of intraabdominal injury.

Methods: This was an observational analysis of prospectively collected data on 45 patients who presented with an abdominal seat belt sign to a level 1 trauma center following a motor vehicle accident between July 2004 and December 2007. The patients were evaluated by computed tomography (CT) scans or ultrasonography (FAST), depending on their hemodynamic stability.

Association of pathologic diagnoses with clinical findings in chronic endometritis.

Objective: To investigate the association of chronic endometritis (CE) with abnormal uterine bleeding, chronic pelvic pain, human immunodeficiency virus (HIV) infection, genital tract infection and salpingitis.

Study Design: In this retrospective study, specimens obtained from endometrial biopsy, dilatation and curettage or hysterectomy were identified. A total of 123 patients with CE and 177 without CE who were used as controls were included in the study.

The influence of work personality on job satisfaction: incremental validity and mediation effects.

Drawing from recent developments regarding the contextual nature of personality (e.g., D.

Stochastic analysis of stepwise fluorescence quenching reactions on single-walled carbon nanotubes: single molecule sensors.

The 1D quantum confinement of photogenerated excitons in single-walled carbon nanotubes (SWNT) can amplify the detection of molecular adsorption to where single-molecule discrimination is realizable, even from within living cells and tissues. Toward this aim, we present a type 1 collagen film, similar to those used as 3D cell scaffolds for tissue engineering, containing embedded SWNT capable of reporting single-molecule adsorption of quenching molecules. We utilize hidden Markov modeling to link single-molecule adsorption events to rate constants for H2O2, H+, and Fe(CN)6(3-).

Vulvar diseases of the tropics: a review.

With the increasing immigrant population in the United States, it is imperative for clinicians in the United States to become versed in tropical vulvar conditions uncommon to indigenous members of developed nations that may present to their practice. Travelers to tropical areas may also contract diseases that occur in these areas, which may manifest as a vulvar condition and mimic a sexually transmitted disease. A brief review is presented.

Combination of spectroscopic methods: in situ NMR and UV/Vis measurements to understand the formation of group 4 metallacyclopentanes from the corresponding metallacyclopropenes.

By reaction of the dichloride rac-(ebthi)HfCl(2) [ebthi = 1,2-ethylene-1,1'-bis(eta(5)-tetrahydroindenyl)] with lithium in the presence of bis(trimethylsilyl)acetylene, the hafnacyclopropene rac-(ebthi)Hf(eta(2)-Me(3)SiC(2)SiMe(3)) (1-Hf) was obtained. The reaction of the blue-green complex 1-Hf with an excess of ethylene at room temperature leads by insertion of the olefin to the yellow-green hafnacyclopentene 2-Hf which is only stable in solution and eliminates the alkyne at 100 degrees C under ethylene to form the corresponding yellow hafnacyclopentane 3-Hf, which was characterized by X-ray crystal structure analysis. The reaction of 1-Hf to give stepwise via 2-Hf the complex 3-Hf was investigated in detail and compared to the formation and stability of the corresponding zirconacyclopropene 1-Zr, zirconacyclopentene 2-Zr, and zirconacyclopentane 3-Zr.

Size-dependent cellular uptake and expulsion of single-walled carbon nanotubes: single particle tracking and a generic uptake model for nanoparticles.

The cellular uptake and expulsion rates of length-fractionated single-walled carbon nanotubes (SWNT) from 130 to 660 nm in NIH-3T3 cells were measured via single particle tracking of their intrinsic photoluminescence. We develop a quantitative model to correlate endocytosis rate with nanoparticle geometry that accurately describes this data set and also literature results for Au nanoparticles. The model asserts that nanoparticles cluster on the cell membrane to form a size sufficient to generate a large enough enthalpic contribution via receptor ligand interactions to overcome the elastic energy and entropic barriers associated with vesicle formation.

Multimodal optical sensing and analyte specificity using single-walled carbon nanotubes.

Nanoscale sensing elements offer promise for single-molecule analyte detection in physically or biologically constrained environments. Single-walled carbon nanotubes have several advantages when used as optical sensors, such as photostable near-infrared emission for prolonged detection through biological media and single-molecule sensitivity. Molecular adsorption can be transduced into an optical signal by perturbing the electronic structure of the nanotubes.

The syringe gap: an assessment of sterile syringe need and acquisition among syringe exchange program participants in New York City.

Background: Programmatic data from New York City syringe exchange programs suggest that many clients visit the programs infrequently and take few syringes per transaction, while separate survey data from individuals using these programs indicate that frequent injecting - at least daily - is common. Together, these data suggest a possible "syringe gap" between the number of injections performed by users and the number of syringes they are receiving from programs for those injections.

Methods: We surveyed a convenience sample of 478 injecting drug users in New York City at syringe exchange programs to determine whether program syringe coverage was adequate to support safer injecting practices in this group.

Simultaneous determination and confirmation of melamine and cyanuric acid in animal feed by zwitterionic hydrophilic interaction chromatography and tandem mass spectrometry.

We developed a new method to analyze animal feed and feed ingredients for melamine and cyanuric acid. The method is capable of extracting and detecting both melamine and cyanuric acid in a single procedure, whether present as free compounds or bound together as the melamine:cyanurate complex. A novel chromatographic system based on zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) columns enables separation and detection of both compounds in one run.

Object-based selection in the Baylis and Driver (1993) paradigm is subject to space-based attentional modulation.

Three experiments re-examined Baylis and Driver's (1993) strong evidence for object-based selection, that making relative apex location judgments is harder between two objects than within a single object, with object (figure-ground) segmentation determined solely by color-based perceptual set. Using variations of the Baylis and Driver paradigm, the experiments replicated a two-object cost. However, they also showed a large part of the two-object cost to be attributable to space-based factors, though there remained an irreducible cost consistent with 'true' object-based selection.

Mediastinal teratoma as a rare cause of hydrops fetalis and death: report of 3 cases.

Background: Congenital mediastinal teratomas are rare and may present with nonimmune hydrops. The lesion may be misinterpreted on ultrasound.

Cases: A 21-year-old woman, gravida 2, para 0111, was evaluated at 19 4/7 weeks of gestation for suspected fetal death.

Adnexal torsion leading to death of an infant.

Background: Torsion of the uterine adnexa is an uncommon occurrence in infants, but when it does occur, the consequences may be catastrophic.

Case: A 4-month-old female presented with sudden cardiac and respiratory arrest. There were no prior signs of illness.

Trinuclear rhodium complexes and their relevance for asymmetric hydrogenation.

Various trinuclear rhodium complexes of the type [Rh(3)(PP)(3)(mu(3)-OH)(x)(mu(3)-OMe)(2-x)]BF(4) (where PP = Me-DuPhos, dipamp, dppp, dppe; different ligands and mu-bridging anions) are presented, which are formed upon addition of bases such as NEt(3) to solvate complexes [Rh(PP)(solvent)(2)]BF(4). They were extensively characterized by X-ray diffraction and NMR spectroscopy ((103)Rh, (31)P, (13)C, (1)H). Their in situ formation resulting from basic additives (NEt(3)) or basic prochiral olefins (without addition of another base) can cause deactivation of the asymmetric hydrogenation.

Evaluation of the renal effects of experimental feeding of melamine and cyanuric acid to fish and pigs.

Objective: To determine whether renal crystals can be experimentally induced in animals fed melamine or the related triazine compound cyanuric acid, separately or in combination, and to compare experimentally induced crystals with those from a cat with triazine-related renal failure.

Animals: 75 fish (21 tilapia, 24 rainbow trout, 15 channel catfish, and 15 Atlantic salmon), 4 pigs, and 1 cat that was euthanatized because of renal failure.

Procedures: Fish and pigs were fed a target dosage of melamine (400 mg/kg), cyanuric acid (400 mg/kg), or melamine and cyanuric acid (400 mg of each compound/kg) daily for 3 days and were euthanatized 1, 3, 6, 10, or 14 days after administration ceased.

Among older adults, the responsiveness of self-rated health to changes in Charlson comorbidity was moderated by age and baseline comorbidity.

Objective: To examine the impact of changes in comorbidity--as measured by the Charlson comorbidity index--on self-rated health in a large sample of community-dwelling elderly over a 1-year period, and to examine the differential effects of changes in specific Charlson diagnostic categories.

Study Design And Setting: Longitudinal survey data on self-rated health were linked with Medicare inpatient, outpatient, and physician visit data for 30,535 U.S.

Medical and support service utilization in a medical program targeting marginalized HIV-infected individuals.

Background: One strategy to facilitate HIV health care services utilization is to incorporate support services with medical services. We developed a program that delivers HIV medical care and support services to marginalized people, and evaluated the association between support and medical services utilization.

Methods: We extracted data on 218 newly enrolled program participants 3 months prior to through 6 months after program enrollment, and analyzed associations between support and medical services.

Delivering care out of the box: the evolution of an HIV harm reduction medical program.

Disparities in HIV health care continue to exist among New York City's marginalized populations. We describe the evolution and development of a unique collaborative program that blends harm reduction and medical care. This program addresses disparities and needs of a particularly marginalized population: unstably housed substance users with HIV infection.

Does the presence of vaginitis on a Pap smear correlate with clinical symptoms in the patient?

Objective: To investigate the association of the finding of trichomoniasis, candidiasis or bacterial vaginosis (BV) on Pap smear with possible symptoms, findings on the clinical examination or treatment that the patient received shortly before or shortly after the results of the Pap smear.

Study Design: Cases were selected from the Department of Pathology, UMDNJ-University Hospital, Newark. Retrospective chart review of patients who had a diagnosis of Trichomonas or Candida infection or bacterial vaginosis on Pap smear was performed.