Do forage production and ingestive behavior of locally adapted sheep differ from an irrigated silvopastoral system to an irrigated monoculture in the semi-arid region?

This study aimed to evaluate forage production and ingestive behavior of Morada Nova sheep in an intensive system with capim-tamani grass in both monoculture and silvopastoral irrigated systems in the Semi-arid region. Eighteen adult sheep, approximately 3 years old, with an average body weight of 26.8 ± 4.

Neoantigen-specific cytotoxic Tr1 CD4 T cells suppress cancer immunotherapy.

CD4 T cells can either enhance or inhibit tumour immunity. Although regulatory T cells have long been known to impede antitumour responses, other CD4 T cells have recently been implicated in inhibiting this response. Yet, the nature and function of the latter remain unclear.

Carcass traits and morphometry, typification of the Longissimus dorsi muscle and non-carcass components of hair lambs: can biscuit bran completely replace corn? A machine learning approach.

Biscuit bran (BB) is a co-product with worldwide distribution, with Brazil as the second largest cookie producer in the world with 1,157,051 tons. We evaluate the impact of completely replacing corn with BB on the characteristics and morphometry of carcass of purebred and crossbred Morada Nova lambs using machine learning techniques as an auxiliary method. Twenty male lambs from two genetic groups (GG) were used: purebred red-coated Morada Nova (MNR) and crossbred MNR × white-coated Morada Nova (MNF1).

Effects of by-product from biscuit industry on performance and carcass traits of fattening Morada Nova lambs.

This research evaluated the effects of using by-product from biscuit industry (BBI) in the diet of fattening lambs of Morada Nova breed. Lamb growth performances and carcass traits were investigated. Twenty Morada Nova breed male lambs body weight (BW) 17.

Changes on the physicochemical and fatty acid profile of meat induced by inclusion of biscuit bran in lamb diet.

The animal feed science is in constant search for new products that bring economic return, without harming the quality of the final product. This study aimed to evaluate the effect of increasing levels of substitution of corn by biscuit bran in lamb diet upon the fatty acid profile of its meat and its physicochemical and sensory characteristics. Twenty-four male lambs divided in four treatments were used.

Nutritional effects and feeding behavior in ewes fed with biscuit bran and cashew nut bran, with different energy levels.

This research evaluated the effects of biscuit bran and cashew nut bran as energy source and additional energy level on intake, digestibility, feeding behavior, energy partitioning, N balance, and blood parameters on ewes. Twenty Morada Nova cull ewes breed (average age of 3 years old and initial body weight of 30.1 ± 3.

Bioeconomic analysis of total replacement of corn grain with by-product from biscuit manufacture for purebred and crossbred Morada Nova lambs in feedlot system in the Brazilian semi-arid region.

The economic feasibility of replacing corn grain with by-product from biscuit manufacture (BBM) as an energy source in the diet for purebred and crossbred native lambs raised in feedlot was evaluated. Four production systems were developed based on nutritional management and genetic groups: (PS) purebred native lambs fed a conventional diet based on corn and soybean meal; (PS) purebred native lambs fed a diet with BBM replacing 100% of the corn; (PS) crossbred native lambs fed a conventional diet based on corn and soybean meal; and (PS) crossbred native lambs fed a diet with BBM replacing 100% of the corn in the diet. The extrapolation was performed for a module of 24 Morada Nova (MN) breed lambs (12 MN red (MNR) and 12 ½MNR × ½MN white lambs)) with an initial body weight of 18.

Feeding restriction in the pre and postpartum period of hair ewes raised in the semi-arid region: implications on performance and carcass traits of the progeny.

Feed restriction (FR) occurs commonly in sheep production systems in the Brazilian semi-arid region and can cause physiological changes in the progeny. We assessed the effects of the FR pre and postnatal on the performance and carcass traits of Morada Nova lambs. Twenty-four lambs born from a group of 68 ewes were distributed in three treatments of 8 replicates as follows: ewes fed ad libitum pre and postnatal (AL-AL); postnatal restriction (POSTN-R): included lambs born from ewes fed ad libitum in the last third of pregnancy with FR postnatal; and prenatal feeding restriction (PREN-AL): comprised lambs born from ewes subjected to FR in the last third of pregnancy but ad libitum postnatal.

Effects of replacing corn with by-product from biscuit manufacture on feed intake, digestibility, nitrogen balance, ingestive behavior, and growth performance of pure or crossbred Morada Nova sheep.

An experiment was carried out to evaluate the effects of replacing corn with by-product from biscuit manufacture (BBM) on feed intake, digestibility, nitrogen balance, ingestive behavior, and growth performance of pure or crossbred Morada Nova sheep. Twenty-four non-castrated male lambs from two genetic groups (GG) were used: purebred red-coated Morada Nova (RMN) and red-coated Morada Nova x white-coated Morada Nova. Each GG had 12 lambs with an initial body weight (BW) of 15.

ClinGen Variant Curation Interface: a variant classification platform for the application of evidence criteria from ACMG/AMP guidelines.

Background: Identification of clinically significant genetic alterations involved in human disease has been dramatically accelerated by developments in next-generation sequencing technologies. However, the infrastructure and accessible comprehensive curation tools necessary for analyzing an individual patient genome and interpreting genetic variants to inform healthcare management have been lacking.

Results: Here we present the ClinGen Variant Curation Interface (VCI), a global open-source variant classification platform for supporting the application of evidence criteria and classification of variants based on the ACMG/AMP variant classification guidelines.

Sheep meat production in the Brazilian semi-arid region: crossing between indigenous breeds.

Investigations in the Brazilian semi-arid region evaluating the performance and carcass traits of sheep of indigenous breeds and their crossings have been performed; however, these studies use exotic breeds which produce precocious lambs with heavier carcasses, but not adapted to climatic conditions and the extensive production system, jeopardizing the sustainability of the sheep production system. We crossed between three indigenous breeds: Morada Nova (MN - maternal breed); Rabo Largo (RL - paternal breed), and Santa Inês (SI - paternal breed) with the objective of evaluating the effect of genotype and sex on the performance and carcass traits of purebred and crossbred animals. A total of 30 lambs, males and females, reared in a semi-intensive system were evaluated.

Proposal for a New Fixation Method for Pauwels Type III Femoral Neck Fracture-Metaphyseal Stem: A Finite-Element Analysis.

Objective: To evaluate the biomechanical behavior of a metaphyseal stem specifically designed for the fixation of Pauwels type-III femoral neck fractures using finite-element analysis.

Methods: Three different constructions were studied: the dynamic hip screw with a superior anti-rotation screw (DHS + ARS), multiple cannulated screws in an inverted triangle configuration (ASNIS), and the Metaphyseal Nailing System (MNS), a new implant developed by the authors. Vertical and total displacement, localized and total maximum and minimum principal, and the Von Mises peak stresses were evaluated.

NASA GeneLab RNA-seq consensus pipeline: standardized processing of short-read RNA-seq data.

With the development of transcriptomic technologies, we are able to quantify precise changes in gene expression profiles from astronauts and other organisms exposed to spaceflight. Members of NASA GeneLab and GeneLab-associated analysis working groups (AWGs) have developed a consensus pipeline for analyzing short-read RNA-sequencing data from spaceflight-associated experiments. The pipeline includes quality control, read trimming, mapping, and gene quantification steps, culminating in the detection of differentially expressed genes.

Clinical Accuracy and Impact of Plasma Cell-Free DNA Fungal Polymerase Chain Reaction Panel for Noninvasive Diagnosis of Fungal Infection.

Background: Invasive fungal infection (IFI) is a growing cause of morbidity and mortality in oncology and transplant patients. Diagnosis of IFI is often delayed due to need for invasive biopsy and low sensitivity of conventional diagnostic methods. Fungal cell-free DNA (cfDNA) detection in plasma is a novel testing modality for the noninvasive diagnosis of IFI.

Comprehensive Multi-omics Analysis Reveals Mitochondrial Stress as a Central Biological Hub for Spaceflight Impact.

Spaceflight is known to impose changes on human physiology with unknown molecular etiologies. To reveal these causes, we used a multi-omics, systems biology analytical approach using biomedical profiles from fifty-nine astronauts and data from NASA's GeneLab derived from hundreds of samples flown in space to determine transcriptomic, proteomic, metabolomic, and epigenetic responses to spaceflight. Overall pathway analyses on the multi-omics datasets showed significant enrichment for mitochondrial processes, as well as innate immunity, chronic inflammation, cell cycle, circadian rhythm, and olfactory functions.

Sheep Methane Emission on Semiarid Native Pasture-Potential Impacts of Either Zinc Sulfate or Propylene Glycol as Mitigation Strategies.

The aim of this study was to evaluate the effects of Zinc sulfate and propylene glycol (PG) on methane (CH) emission, nutrient intake, digestibility, and production in sheep grazing on a native (Brazilian semi-arid savannah) pasture during the rainy season (from March to June 2014). Fifteen mixed Santa Inês sheep, all non-castrated males, with initial body weight of 19.8 ± 1.

LitGen: Genetic Literature Recommendation Guided by Human Explanations.

As genetic sequencing costs decrease, the lack of clinical interpretation of variants has become the bottleneck in using genetics data. A major rate limiting step in clinical interpretation is the manual curation of evidence in the genetic literature by highly trained biocurators. What makes curation particularly time-consuming is that the curator needs to identify papers that study variant pathogenicity using different types of approaches and evidences-e.

Increasing Clinical Trial Accrual via Automated Matching of Biomarker Criteria.

Successful implementation of precision oncology requires both the deployment of nucleic acid sequencing panels to identify clinically actionable biomarkers, and the efficient screening of patient biomarker eligibility to on-going clinical trials and therapies. This process is typically performed manually by biocurators, geneticists, pathologists, and oncologists; however, this is a time-intensive, and inconsistent process amongst healthcare providers. We present the development of a feature matching algorithmic pipeline that identifies patients who meet eligibility criteria of precision medicine clinical trials via genetic biomarkers and apply it to patients undergoing treatment at the Stanford Cancer Center.

Effects of Zinc Sulfate or Propylene Glycol on Intake, Digestibility, and Forage Selection by Grazing Sheep in a Semi-Arid Region During the Rainy Season.

The objective of this study was to determine intake, nutrient availability, and animal selection of major forage species in sheep supplemented with zinc sulfate or propylene glycol in -native pastures during the rainy season. Twenty-four mixed Santa Inês sheep, all non-castrated males, with initial weight of 19.3 ± 2.

High-throughput Sequencing of Subcutaneous Panniculitis-like T-Cell Lymphoma Reveals Candidate Pathogenic Mutations.

Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a malignant primary cutaneous T-cell lymphoma that is challenging to distinguish from other neoplastic and reactive panniculitides. In an attempt to identify somatic variants in SPTCL that may be diagnostically or therapeutically relevant, we performed both exome sequencing on paired tumor-normal samples and targeted sequencing of hematolymphoid-malignancy-associated genes on tumor biopsies. Exome sequencing was performed on skin biopsies from 4 cases of skin-limited SPTCL, 1 case of peripheral T-cell lymphoma, not otherwise specified with secondary involvement of the panniculus, and 2 cases of lupus panniculitis.

Genomic Evidence for Local Adaptation of Hunter-Gatherers to the African Rainforest.

African rainforests support exceptionally high biodiversity and host the world's largest number of active hunter-gatherers [1-3]. The genetic history of African rainforest hunter-gatherers and neighboring farmers is characterized by an ancient divergence more than 100,000 years ago, together with recent population collapses and expansions, respectively [4-12]. While the demographic past of rainforest hunter-gatherers has been deeply characterized, important aspects of their history of genetic adaptation remain unclear.

Structural Variation Detection by Proximity Ligation from Formalin-Fixed, Paraffin-Embedded Tumor Tissue.

The clinical management and therapy of many solid tumor malignancies depends on detection of medically actionable or diagnostically relevant genetic variation. However, a principal challenge for genetic assays from tumors is the fragmented and chemically damaged state of DNA in formalin-fixed, paraffin-embedded (FFPE) samples. From highly fragmented DNA and RNA there is no current technology for generating long-range DNA sequence data as is required to detect genomic structural variation or long-range genotype phasing.

Detection and Surveillance of Bladder Cancer Using Urine Tumor DNA.

Current regimens for the detection and surveillance of bladder cancer are invasive and have suboptimal sensitivity. Here, we present a novel high-throughput sequencing (HTS) method for detection of urine tumor DNA (utDNA) called utDNA CAPP-Seq (uCAPP-Seq) and apply it to 67 healthy adults and 118 patients with early-stage bladder cancer who had urine collected either prior to treatment or during surveillance. Using this targeted sequencing approach, we detected a median of 6 mutations per patient with bladder cancer and observed surprisingly frequent mutations of the promoter (46%), suggesting these mutations represent a useful biomarker for detection of bladder cancer.