Publications by authors named "Helene Marie Roux"
Cell Rep Methods
April 2023
Article Synopsis
- Unintegrated HIV DNA makes up 20-35% of the total viral DNA in infected patients, with linear forms (unintegrated linear DNAs or ULDs) being crucial for integration and viral replication.
- The detection of ULDs is challenging due to the limitations of existing methods, which lack specificity and sensitivity.
- A new technology called DUSQ, combining linker-mediated PCR and next-generation sequencing, has been developed for ultra-sensitive quantification of ULDs and can track pre-integrative latency in HIV-1 infected patients, with potential applications for other rare DNA molecules.
