A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS Method-Application to , , , and .

Concentrated bud macerates (CBMs) are obtained from meristematic tissues such as buds and young shoots by maceration in a solvent composed of glycerin, water and ethanol (1/1/1/, /). Their traditional utilization in gemmotherapy has gained interest in the past years, and the knowledge of their chemical characterization can provide commercial arguments, particularly to secure their quality control. Therefore, an optimized method for phytochemical analysis including glycerol removal by a preliminary solid phase extraction (SPE) followed by compound identification using high performance liquid chromatography coupled with ultra-violet and tandem mass detectors (HPLC-UV-MS) was developed.

New Metabolites Isolated from a Laurencia obtusa Population Collected in Corsica.

The chemical investigation of an ethyl acetate extract (EtOAc) obtained from , collected in Corsica, allowed for the identification of three new compounds (, , and ) and six known compounds. Compounds to were isolated and fully characterized by a detailed spectroscopic analysis. Compounds and are two C-acetogenins sharing the same ring system: a tetrahydropyran linked by a methylene to a tetrahydrofuran ring.

Discrimination and Characterization of Two Mediterranean Species from the Laurencia Complex (Rhodomelacea) Using an NMR-Based Metabolomic Approach.

Generic and specific determination among the Laurencia complex is a challenging task. DNA barcoding combined with phenotypic investigations are mandatory for species differentiation. In this study, two morphologically different members of the Laurencia complex were investigated using untargeted H-NMR-based metabolomics.

Chemical Composition of Laurencia obtusa Extract and Isolation of a New C-Acetogenin.

A new C-acetogenin, sagonenyne (), exhibiting an unusual single tetrahydropyran ring was isolated from an ethyl acetate extract of collected on the Corsican coastline. Its structure was established by detailed NMR spectroscopic analysis, mass spectrometry, and comparison with literature data. Twenty-three known compounds were identified in the same extract by means of column chromatography steps, using a C-NMR computer aided method developed in our laboratory.