A comparison of three methods for preparing centered platforms around separated instruments in curved canals.

This study compared three methods for creating the most centered staging platform (SP) around separated instruments (SI) in curved canals. Green .04 ProFiles, notched at D(3), were separated in the apical third of 42 mesiobuccal canals of maxillary and mandibular molars.

Investigating structural changes induced by nucleotide binding to RecA using difference FTIR.

Nucleotide binding to RecA results in either the high-DNA affinity form (Adenosine 5'-triphosphate (ATP)-bound) or the more inactive protein conformation associated with a lower affinity for DNA (Adenosine 5'-diphosphate (ADP)-bound). Many of the key structural differences between the RecA-ATP and RecA-ADP bound forms have yet to be elucidated. We have used caged-nucleotides and difference FTIR in efforts to obtain a comprehensive understanding of the molecular changes induced by nucleotide binding to RecA.