Publications by authors named "Helena Maria D Danielsen"
The cell membrane is crucial for cell survival, and ensuring its integrity is essential as the cell experiences injuries throughout its entire life cycle. To prevent damage to the membrane, cells have developed efficient plasma membrane repair mechanisms. These repair mechanisms can be studied by combining confocal microscopy and nanoscale thermoplasmonics to identify and investigate the role of key proteins, such as annexins, involved in surface repair in living cells and membrane model systems.
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- Maintaining the cell plasma membrane's integrity is essential for cell survival, as damaged membranes can lead to issues with calcium influx and impede drug delivery.
- The study demonstrates a method to create controlled nanoscopic holes in the plasma membrane using laser-induced heating of gold nanostructures, allowing for the study of membrane repair mechanisms.
- Annexin V is identified as a key player in reshaping the membrane around these holes, providing insights into cellular responses to membrane injuries and the effectiveness of photothermal therapy.
Novel RNA-based technologies provide an avenue of possibilities to control the regulation of gene expression in cells. To realize the full potential of small interfering RNA (siRNA)-based therapy, efficient delivery vehicles and novel strategies for triggering release from carrier vehicles have to be developed. Gold nanoparticles (AuNPs) with sizes of ∼50-150 nm have the ability to accumulate in tumor tissue and can be transported across the membrane by endocytosis.
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