Publications by authors named "Helen E Dahlke"

Groundwater aquifers worldwide experience unsustainable depletion, compounded by population growth, economic development, and climate forcing. Managed aquifer recharge provides one tool to alleviate flood risk and replenish groundwater. However, concerns grow that intentional flooding of farmland for groundwater recharge, a practice known as Ag-MAR, may increase the leaching of pesticides and other chemicals into groundwater.

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Agricultural managed aquifer recharge (Ag-MAR) is a promising approach to replenish groundwater resources using flood water and cropland as spreading grounds. However, site selection, particularly the layering of sediment deposits in the subsurface, can greatly influence Ag-MAR efficacy as it controls water flow and solute transport in the vadose zone. In this study, we use the HYDRUS-1D software to simulate water flow and solute transport from the land surface to the groundwater table in three vadose zone profiles (LS, MS, HS) characterized by differing fractions of sand (44 %, 47 %, and 64 %).

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It has been recently demonstrated that free DNA tracers have the potential in tracing water flow and contaminant transport through the vadose zone. However, whether the free DNA tracer can be used in flood irrigation area to track water flow and solute/contaminant transport is still unclear. To reveal the infiltration process and retention mechanisms of surface applied free DNA tracer through soil under flood irrigation, we tested the fate and transport behavior of surface applied free DNA tracers through packed saturated sandy soil columns with a 10 cm water head mimicking flood irrigation.

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Nitrate (NO) leaching is a serious health and ecological concern in global agroecosystems, particularly those under the application of agricultural-managed aquifer recharge (Ag-MAR); however, there is an absence of information on microbial controls affecting NO leaching outcomes. We combine natural dual isotopes of NO (N/N and O/O) with metagenomics, quantitative polymerase chain reaction (PCR), and a threshold indicator taxa analysis (TITAN) to investigate the activities, taxon profiles, and environmental controls of soil microbiome associated with NO leaching at different depths from Californian vineyards under Ag-MAR application. The isotopic signatures demonstrated a significant priming effect ( < 0.

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Agricultural managed aquifer recharge (Ag-MAR, on-farm recharge), where farmland is flooded with excess surface water to intentionally recharge groundwater, has received increasing attention by policy makers and researchers in recent years. However, there remain concerns about the potential for Ag-MAR to exacerbate nitrate (NO) contamination of groundwater, and additional risks, such as greenhouse gas emissions and crop tolerance to prolonged flooding. Here, we conducted a large-scale, replicated winter groundwater recharge experiment to quantify the effect of Ag-MAR on soil N biogeochemical transformations, potential NO leaching to groundwater, soil physico-chemical conditions, and crop yield.

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Although multiple experimental studies have proven the use of free synthetic DNA as tracers in hydrological systems, their quantitative fate and transport, especially through the vadose zone, is still not well understood. Here we simulate the water flow and breakthrough of deuterium (D) and one free synthetic DNA tracer from a 10-day experiment conducted in a transient variably saturated 1m 10° sloped lysimeter using the HYDRUS-2D software package. Recovery and breakthrough flux of D (97.

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Mountain regions in arid and semi-arid climates, such as California, are considered particularly sensitive to climate change because global warming is expected to alter snowpack storage and related surface water supply. It is therefore important to accurately capture snowmelt processes in watershed models for climate change impact assessment. In this study we use the Soil and Water Assessment Tool (SWAT) to estimate projected changes in snowpack and streamflow in four alpine tributaries to the agriculturally important but less studied southern Central Valley, California.

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Balancing ecological and human water needs often requires characterizing key aspects of the natural flow regime and then predicting ecological response to flow alterations. Flow metrics are generally relied upon to characterize long-term average statistical properties of the natural flow regime (hydrologic baseline conditions). However, some key aspects of hydrologic baseline conditions may be better understood through more complete consideration of continuous patterns of daily, seasonal, and inter-annual variability than through summary metrics.

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Poly(lactic-co-glycolic acid) (PLGA) particle carriers of synthetic DNA have recently received increased attention for environmental applications due to their biodegradability, customizability, and nearly limitless number of uniquely identifiable "labels". In this paper, we present methodologies for the preparation of DNA-labeled particles, control of particle size, extraction of DNA-labels, and analysis via quantitative polymerase chain reaction (qPCR). Characterization and analysis of the DNA-labeled particles reveal spherical particles of diameters ranging from 60 to 1000 nm, with consistent zeta potentials around -45 mV, that are stable to aggregation, even in the presence of concentrated mono- and divalent cations.

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Escherichia coli (E. coli) level in streams is a public health indicator. Therefore, being able to explain why E.

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