Publications by authors named "Helen C Baxter"
Dental handpieces (DHPs) become biofouled internally with patient derived material that is difficult to access for removal and inactivation. This study undertook a quantitative and qualitative investigation of protein contamination of internal components from three different types of DHP: the turbine, slow speed contra-angle and surgical. Eluates from the high speed turbine, low speed spray channels and surgical gear were assayed for protein using an orthophthaldehyde assay.
View Article and Find Full Text PDFA synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.
View Article and Find Full Text PDFArticle Synopsis
- - The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) involves clinical observations and a positive test for 14-3-3 proteins in cerebrospinal fluid, though the exact role of these proteins in CJD is unclear.
- - Researchers created a mutant mouse without the 14-3-3gamma protein to explore its role in diseases like CJD, finding no anatomical or behavioral changes and unchanged levels of other 14-3-3 proteins in the brains.
- - After inoculating both mutant and normal mice with the scrapie strain, there were no differences in survival rates, suggesting that the absence of 14-3-3gamma does not influence the progression of CJD or