Publications by authors named "Helaine G S Vieira"
Benznidazole (BZ) is the trypanocidal compound of choice for Chagas disease, a neglected tropical disease in the Americas. However, this drug often fails to cure the infection. The regulation of gene expression in Trypanosoma cruzi, the causative agent of Chagas disease, is based on post-transcriptional mechanisms.
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- - Nanopore RNA sequencing is a promising technique for identifying various RNA modifications, including N-methyladenosine, pseudouridine (Ψ), and 2'-O-methylation (Nm), through distinctive base-calling error patterns.
- - The study identifies both known and novel Ψ modification sites across different RNA types in yeast, highlighting a specific modification in mitochondrial rRNA that is dependent on the enzyme Pus4.
- - Researchers created a software tool called nanoRMS to quantify the levels of RNA modifications at individual sites by analyzing altered current intensity in nanopore sequencing data, showcasing the potential to uncover significant insights into RNA modification dynamics under stress conditions.
Trypanosoma cruzi, the causative agent of Chagas disease, has a complex life cycle that requires the adaptation to different environments. In the absence of traditional mechanisms for regulation of gene expression, this parasite relies on posttranscriptional control events, which allow the progression of its life cycle in different hosts and stress conditions. In this context, different stress conditions trigger the aggregation of RNA-binding proteins and their target mRNAs into cytoplasmic foci known as RNA granules, which act as RNA-sorting centers.
View Article and Find Full Text PDFWe characterize a novel human cohesinopathy originated from a familial germline mutation of the gene encoding the cohesin subunit STAG2, which we propose to call -related X-linked Intellectual Deficiency. Five individuals carry a p.Ser327Asn (c.
View Article and Find Full Text PDFTrypanosoma cruzi, the causative agent of Chagas disease, is extremely resistant to ionizing radiation, enduring up to 1.5 kGy of gamma rays. Ionizing radiation can damage the DNA molecule both directly, resulting in double-strand breaks, and indirectly, as a consequence of reactive oxygen species production.
View Article and Find Full Text PDFSeveral genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and shown to play an important role in Ub-dependent processes, little is known about their role in S. mansoni .
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