Evaluation of amlodipine against strains of spp. in planktonic cells, developing biofilms and mature biofilms.

To evaluate the antifungal activity of amlodipine against strains of spp. and to its possible mechanism of action. Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index.

Biosurfactant complexed with arginine has antibiofilm activity against methicillin-resistant .

The present study investigated the antimicrobial effectiveness of a rhamnolipid complexed with arginine (RLMIX_Arg) against planktonic cells and biofilms of methicillin-resistant (MRSA). Susceptibility testing was performed using the Clinical & Laboratory Standards Institute protocol: M07-A10, checkerboard test, biofilm in plates and catheters and flow cytometry were used. RLMIX_Arg has bactericidal and synergistic activity with oxacillin.

Synergistic effect of hydralazine associated with triazoles on spp. in planktonic cells.

To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against spp. and the action mechanism. We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism.

Antifungal activity of dexamethasone against fluconazole-resistant and its activity against biofilms.

The present study investigated the antifungal action of dexamethasone disodium phosphate (Dex). Susceptibility testing was performed using the Clinical & Laboratory Standards Institute protocol; M27-A3, checkerboard test and biofilm were evaluated with two isolates of , hyphal production test, molecular docking analysis and flow cytometry analysis. Dex and fluconazole (FLC) together had a synergistic effect.

Synergistic activity of dobutamine combined with azoles and its mechanism of action against strains of .

To evaluate the antifungal effect of dobutamine against as well as its synergism with azoles and its action on biofilm. The M27-A3 protocol and flow cytometry were used for elucidation of the possible mechanism of action. The tested isolates presented MICs ranging from 2 to 32 μg/ml for dobutamine, with fungistatic effect.

Synergistic activity of diclofenac sodium with oxacillin against planktonic cells and biofilm of methicillin-resistant strains.

To evaluate the activity of diclofenac sodium and synergism with oxacillin against clinical strains of SARM in plactonic cells, antibiofilm and biofilm. Synergism activity was assessed using the fractional inhibitory concentration index and its possible mechanism of action by flow cytometry. The synergistic activity of diclofenac sodium with oxacillin was observed against plactonic cells, antibiofilm and in biofilm formed from clinical methicillin-resistant strains.

Synergistic effects of ketamine and azole derivatives on spp. resistance to fluconazole.

The emergence of spp. with resistance to antifungal molecules, mainly the azole class, is an increasing complication in hospitals around the globe. In the present research, we evaluated the synergistic effects of ketamine with two azole derivatives, itraconazole and fluconazole, on strains of spp.

Synergistic anticandidal activity of etomidate and azoles against clinical fluconazole-resistant isolates.

The purpose of this study was to evaluate the effect of etomidate alone and in combination with azoles on resistant strains of spp. in both planktonic cells and biofilms. The antifungal activity of etomidate was assessed by the broth microdilution test; flow cytometric procedures to measure fungal viability, mitochondrial transmembrane potential, free radical generation and cell death; as well detection of DNA damage using the comet assay.