Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat.

Cultured or cultivated meat, animal muscle, and fat tissue grown in vitro, could transform the global meat market, reducing animal suffering while using fewer resources than traditional meat production and no antimicrobials at all. To ensure the appeal of cultured meat to future customers, cultured fat is essential for achieving desired mouthfeel, taste, and texture, especially in beef. In this work we show the establishment of primary bovine adipose-derived stem cell spheroids in static and dynamic suspension culture.

Methodological concerns underlying a lack of evidence for cultural heterogeneity in the replication of psychological effects.

The multi-site replication study, Many Labs 2, concluded that sample location and setting did not substantially affect the replicability of findings. Here, we examine theoretical and methodological considerations for a subset of the analyses, namely exploratory tests of heterogeneity in the replicability of studies between "WEIRD and less-WEIRD cultures". We conducted a review of literature citing the study, a re-examination of the existing cultural variability, a power stimulation for detecting cultural heterogeneity, and re-analyses of the original exploratory tests.

Activation of the aryl hydrocarbon receptor improves allergen-specific immunotherapy of murine allergic airway inflammation: a novel adjuvant option?

Background: Allergen-specific immunotherapy (AIT) is able to restore immune tolerance to allergens in allergic patients. However, some patients do not or only poorly respond to current treatment protocols. Therefore, there is a need for deeper mechanistic insights and further improvement of treatment strategies.

Seasonal dynamics of the standard test species Lemna sp. in outdoor microcosms.

Lemna L. sp. is a free-floating aquatic macrophyte that plays a key role as a standard test species in aquatic risk assessment for herbicides and other contaminants.

When are people more open to cheating? Economic inequality makes people expect more everyday unethical behavior.

Economic inequality has been found to be associated with increased unethical behavior and an increased acceptance of unethical behavior. In this paper we explored whether higher amounts of perceived inequality lead to an increase in the expectation of unethical behavior. We tested whether people would say that they themselves would engage in more unethical behavior in a context of high compared to low inequality.

Biofabrication's Contribution to the Evolution of Cultured Meat.

Cultured Meat (CM) is a growing field in cellular agriculture, driven by the environmental impact of conventional meat production, which contributes to climate change and occupies ≈70% of arable land. As demand for meat alternatives rises, research in this area expands. CM production relies on tissue engineering techniques, where a limited number of animal cells are cultured in vitro and processed to create meat-like tissue comprising muscle and adipose components.

Shigella virulence protein VirG is a broadly protective antigen and vaccine candidate.

Diarrhea caused by Shigella has been associated with high morbidity and mortality in young children worldwide. There are no licensed vaccines, and those clinically advanced have restricted coverage as they elicit serotype-specific immunity while disease is caused by multiple circulating serotypes. Our group had previously reported a close association between serum antibodies to the Shigella virulence factor VirG (or IcsA) and clinical protection in infected individuals.

Shame and anger differentially predict disidentification between collectivistic and individualistic societies.

In the present research we tested the differential effects of anger versus shame as emotional predictors of ingroup disidentification in one rather collectivistic (Japan) and two rather individualistic societies (Germany, Canada). We tested the idea that individuals cope with socially undesired emotions by disidentifying from their group. Specifically, we predicted that after a group conflict, anger, an undesired emotion in Japan, would elicit disidentification in Japan, whereas shame, an undesired emotion in Canada and Germany, would elicit disidentification in Germany and Canada.

Epidemiology and Characteristics of Gastric Carcinoma in Childhood-An Analysis of Data from Population-Based and Clinical Cancer Registries.

(1) Background: Gastric carcinoma is an exceptionally rare tumor in childhood. Little is known about the etiology, epidemiology, and clinical features of pediatric gastric carcinomas. This analysis aimed to fill this gap by increasing knowledge about the occurrence of gastric carcinoma in childhood.

Leadership in the eye of the beholder: Follower self-esteem is associated with divergent perceptions of leadership ability for dominant and prestigious leaders.

Objective: Drawing from dual-strategies theory, leader-member exchange theory, and several theories of self-esteem, we develop and test hypotheses about how followers' self-esteem predicts their perceptions of dominant and prestigious leaders' leadership ability.

Method: Across four studies (N = 1568), we tested the association between self-esteem and perceptions of leadership ability for dominant and prestigious leaders.

Results: Individuals with high self-esteem perceived greater leadership ability in prestigious leaders than did those with low self-esteem and individuals with low self-esteem perceived greater leadership ability in dominant leaders than did those with high self-esteem.

Digital resources as an aspect of teacher professional digital competence: One term, different definitions - a systematic review.

The term 'digital resources' is increasingly used in educational research to describe the specific knowledge and skills that constitute teachers' professional digital competence. Educational policy documents, including the European Framework for the Digital Competence of Educators (DigCompEdu), deploy the term to reaffirm teachers' need for special skills in using digital resources. However, educational research literature presents inconsistent views of the term, restricting its effective use in further research and the promotion of associated skills among pre-service and in-service teachers.

The (im-)moral scientist? Measurement and framing effects shape the association between scientists and immorality.

Recent years have not only seen growing public distrust in science, but also in the people conducting science. Yet, attitudes toward scientists remain largely unexplored, and the limited body of literature that exists points to an interesting ambivalence. While survey data suggest scientists to be positively evaluated (e.

Genomic distribution and context dependent functionality of novel WRKY transcription factor binding sites.

Background: The WT-boxes NGACTTTN are novel microbe-associated molecular pattern (MAMP)-responsive cis-regulatory sequences. Many of them are uncommon WRKY transcription factor (TF) binding sites.

Results: To understand their functional relevance, a genomic distribution analysis of the 16 possible WT-boxes and a functional analysis of a WT-box rich promoter was done.

The dubious precision and utility of heritability estimates.

Uchiyama et al. question heritability estimates in a convincing manner. We offer additional arguments to further bolster their claims, highlighting methodological issues in heritability coefficients' derivation, their misuse in various contexts, and their potential contributions to exacerbating common erroneous intuitions that have been shown to lead to deleterious social phenomena.

Thermosensitive PLGA-PEG-PLGA Hydrogel as Depot Matrix for Allergen-Specific Immunotherapy.

Allergen-specific immunotherapy (AIT) is the only currently available curative treatment option for allergic diseases. AIT often includes depot-forming and immunostimulatory adjuvants, to prolong allergen presentation and to improve therapeutic efficacy. The use of aluminium salts in AIT, which are commonly used as depot-forming adjuvants, is controversially discussed, due to health concerns and Th2-promoting activity.

Immune-mediated thrombotic thrombocytopenic purpura in a Jehovah's Witness - Effectiveness of incorporating extracorporeal immunoadsorption to caplacizumab, steroids and rituximab.

We report the case of a Jehovah's Witness adolescent patient with immune-mediated thrombotic thrombocytopenic purpura after SARS-Cov2 infection successfully treated without therapeutic plasma exchange (TPE) using caplacizumab, corticosteroids, rituximab, and extracorporeal immunoadsorption (EIA). Further patients for whom TPE is not an option might benefit from this approach.

From Freud to Android: Constructing a Scale of Uncanny Feelings.

The uncanny valley is a topic for engineers, animators, and psychologists, yet uncanny emotions are without a clear definition. Across three studies, we developed an 8-item measure of unnerved feelings, finding that it was discriminable from other affective experiences. In Study 1, we conducted an exploratory factor analysis that yielded two factors; an unnerved factor, which connects to emotional reactions to the uncanny, and a disoriented factor, which connects to mental state changes more distally following uncanny experiences.

Subjective Economic Inequality Decreases Emotional Intelligence, Especially for People of High Social Class.

Across five studies (three preregistered; = 2,481), we investigated two effects as follows: (1) Is higher subjective economic inequality associated with a decreased ability to accurately identify emotions (emotional intelligence)? When inequality is high, people are less focused on others and may thus be less motivated to correctly identify their emotions. (2) Is this main effect of subjective inequality qualified by an interaction with socioeconomic status (SES)? Past research suggests that high SES leads to lower emotional intelligence because people of higher SES are less dependent on others and thus less motivated to identify their emotions. When perceiving higher inequality, high SES individuals should feel even more self-reliant, thereby exacerbating the difference in emotional intelligence between people of low and high SES.

Scaffolds for Cultured Meat on the Basis of Polysaccharide Hydrogels Enriched with Plant-Based Proteins.

The world population is growing and alternative ways of satisfying the increasing demand for meat are being explored, such as using animal cells for the fabrication of cultured meat. Edible biomaterials are required as supporting structures. Hence, we chose agarose, gellan and a xanthan-locust bean gum blend (XLB) as support materials with pea and soy protein additives and analyzed them regarding material properties and biocompatibility.

Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial.

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare.

The Construct of Subjective Economic Inequality.

Economic inequality has been associated with a host of social ills, but most research has focused on objective measures of inequality. We argue that economic inequality also has a subjective component, and understanding the effects of economic inequality will be deepened by considering the ways that people perceive inequality. In an American sample ( = 1,014), we find that some of the key variables that past research has found to correlate with objective inequality also correlate with a subjective measure of inequality.

Seasonal dynamics of the macrophyte test species Myriophyllum spicatum over two years in experimental ditches for population modeling application in risk assessment.

Myriophyllum spicatum is a sediment-rooted, aquatic macrophyte growing submerged, with a wide geographical distribution and high ecological relevance in freshwater ecosystems. It is used in testing and risk assessment for pesticides in water and sediment. Population models enable effects measured under laboratory conditions to be extrapolated to effects expected in the field with time-variable environmental factors including exposure.