Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation.

We report the use of pendant droplet evaporation for exchange of eluents for (1)H nuclear magnetic resonance ((1)H NMR) purposes. Analytes are fed and retained in 500 nL droplets, which are concentrated by evaporation and subsequently redissolved in deuterated solvent. Droplet size is monitored by machine vision (MV), and heating rates are adjusted concordingly to maintain a stable droplet volume.

Tunable ionic mobility filter for depletion zone isotachophoresis.

We present a novel concept of filtering based on depletion zone isotachophoresis (dzITP). In the micro/nanofluidic filter, compounds are separated according to isotachophoretic principles and simultaneously released selectively along a nanochannel-induced depletion zone. Thus, a tunable low-pass ionic mobility filter is realized.

Limits of miniaturization: assessing ITP performance in sub-micron and nanochannels.

The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel.

Single-electrolyte isotachophoresis using a nanochannel-induced depletion zone.

Isotachophoretic separations are triggered at the border of a nanochannel-induced ion-depleted zone. This depletion zone acts as a terminating electrolyte and is created by concentration polarization over the nanochannel. We show both continuous and discrete sample injections as well as separation of up to four analytes.

An efficient method for the fabrication of temperature-sensitive hydrogel microactuators.

In this article a new method for the photolithographical deposition of temperature-sensitive hydrogels is presented. The method can be used in conjunction with standard 365 nm UV-photolithography to accurately dimension and position temperature-sensitive hydrogel microactuators in a highly parallel fashion. A number of characteristics of the hydrogels were investigated.

Stimulus-sensitive hydrogels and their applications in chemical (micro)analysis.

In this tutorial review the use of stimulus-sensitive hydrogels as sensors and actuators for (micro)analytical applications is discussed. The first part of the article is aimed at making the reader familiar with stimulus-sensitive hydrogels, their chemical composition and their chemo-physical behavior. The prior art in the field, that comprises a number of sensors ranging from metal ion-sensitive sensors to antigen-sensitive sensors and a few actuators, is also treated in this part.