Publications by authors named "Heike Smolian"
Article Synopsis
- Mesenchymal stem cells (MSC) from pigs show promise for tissue engineering due to their ability to remain undifferentiated or mature into different mesenchymal tissues.
- A protocol was developed to isolate porcine MSC from bone marrow, with a focus on differentiating them into various tissue types.
- The successful differentiation into bone, fat, and cartilage was confirmed through various methods such as calcium deposition, gene expression analysis, and histological staining.
Objective: An important role in joint and cartilage homeostasis in adults has been demonstrated recently for morphogenetic factors of the transforming growth factor beta family. Therefore, this study was undertaken to investigate the potential of bone morphogenetic proteins (BMPs) in chondrocyte differentiation using current technologies of tissue engineering.
Methods: Complementary DNAs of recombinant human BMPs 2, 4, 5, 6, and 7 were transfected into primary bovine articular chondrocytes.