Rapid detection of important human pathogenic Phleboviruses.

Background: Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae.

Objectives: To develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV).

Study Design: RNA standards were generated and used to test the performance of the assays.