Effect of implementation of a surgical safety checklist on perioperative and postoperative complications at an academic institution in North America.

Objective: To evaluate the efficacy of a surgical safety checklist (SSC) in reducing perioperative and postoperative complications.

Study Design: Before-and-after intervention study.

Animals: Client-owned dogs (n = 633) and cats (n = 44).

Development of the AmpFISTR SEfiler PCR amplification kit: a new multiplex containing the highly discriminating ACTBP2 (SE33) locus.

The AmpFISTR SEfiler kit co-amplifies 11 short tandem repeat loci including SE33 in a single multiplex. After establishing the optimum in primer titration studies, the primer concentrations of all loci in the multiplex were chosen such that the heterozygote peak height ratios of each of the loci were balanced. The combined primer set was then tested to determine the robustness of the multiplex under various conditions.