Complete genome sequence of D1_6, isolated from peat soil.

We present a complete genome of D1_6 isolated from peat swamp forest. The complete genome for the isolate D1_6 was constructed using data from Oxford Nanopore Technologies and Illumina. The genome of D1_6 has a total length of 4,996,151 bp, comprising a chromosome and a plasmid.

Complete genome sequence of potential plant growth-promoting strain AIMST-CREST03 isolated from paddy field bulk soil.

We present the complete genome of a potential plant growth-promoting bacteria isolated from a high-yielding paddy plot. The genome is 3,669,202 bp in size with a GC content of 41%. Annotation predicted 3,327 coding sequences, including several genes required for plant growth promotion.

Complete genome sequence of phage EF_RCK.

A lytic bacteriophage EF_RCK infecting was isolated from a water sample collected in a raw cockle storage container at Taman Ria market, Sungai Petani, Malaysia. The phage has a 57,848-bp double-stranded DNA genome harboring 107 protein-encoding genes and shares 90.9% nucleotide similarity with phage EFKL ( genus).

Complete genome sequence of plant growth-promoting AIMST-CREST02 isolated from bulk soil of a paddy field.

Here, we present the complete genome of a plant growth-promoting strain, AIMST-CREST02 isolated from the bulk soil of a high-yielding paddy plot. The genome is 3,840,451 bp in size with a GC content of 41.25%.

Complete genome sequences of D1_2, a bacterium with antimicrobial properties isolated from peat soil.

is a beneficial lactic acid bacterium commonly studied for its probiotic properties and role in dairy production. Here, we present a complete genome of D1_2, isolated from peat swamp forests. To discover the potential antimicrobial properties, the complete genome of the strain was sequenced and analyzed.

Genome characterization of bacteriophage W2B.

We have successfully characterized the complete genome sequence of the lytic bacteriophage W2B, isolated from the Bunus Sewage Treatment Plant. The lytic phage from the Ningirsuvirus family has a 40,385-bp linear double-stranded DNA genome containing 51 coding sequences (CDSs).

Characterization of the complete mitogenome data of (Lepidoptera: Noctuoidea: Erebidae) from Malaysia.

is a moth species from the genus a member of the Lepidoptera family, Erebidae. Due to their wide variation, this family constitutes the largest described species, however, the mitogenome dataset on the genus is scarce. Hence, the mitochondrial genome dataset of from Malaysia was completely sequenced using the next-generation sequencing technology, Illumina NovaSeq 6000 and analyzed.

Genome Characterization of Enterococcus faecalis Bacteriophage EFKL.

We characterized the complete genome of the lytic Enterococcus faecalis phage EFKL, which was isolated from a sewage treatment plant in Kuala Lumpur, Malaysia. The phage, which was classified in the genus , has a 58,343-bp double-stranded DNA genome containing 97 protein-encoding genes and shares 80.60% nucleotide similarity with phage EF653P5 and phage EF653P3.

Characterization of the nearly complete mitochondrial genome of ochraceous darkies, Selys, 1859 (Odonata: Zygoptera: Euphaeidae) and phylogenetic analysis.

In the present study, the nearly complete mitochondrial genome of was described and its phylogenetic position in the family Euphaeidae was analyzed. Here, we recovered 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs and a partial control region, resulting in a mitogenome length of 15,545bp. All protein-coding genes were initiated by the typical ATN codon except and , which utilizes the TTG codon.

Phenotypic Characterization and Comparative Genomic Analysis of Novel Bacteriophages Isolated from a Tropical Rainforest.

infections across the globe are becoming more challenging to control due to the emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these multidrug-resistant infections. Most phages to date were collected from human-impacted environments.

Complete mitochondrial genome data and phylogenetic analysis of the Great Marquis, (Doubleday, 1848) (Lepidoptera: Nymphalidae: Limenitidinae) from Malaysia.

The Great Marquis or is a butterfly species commonly found in the tropical regions of Asia, America, and Africa. This butterfly is a member of the subfamily Limenitidinae and the classification within this subfamily has been unstable. Here, we report the first complete mitochondrial genome (mitogenome) of sampled from Malaysia.

PhageLeads: Rapid Assessment of Phage Therapeutic Suitability Using an Ensemble Machine Learning Approach.

The characterization of therapeutic phage genomes plays a crucial role in the success rate of phage therapies. There are three checkpoints that need to be examined for the selection of phage candidates, namely, the presence of temperate markers, antimicrobial resistance (AMR) genes, and virulence genes. However, currently, no single-step tools are available for this purpose.

The complete mitochondrial genome data of the Common Rose butterfly, (Lepidoptera, Papilionoidea, Papilionidae) from Malaysia.

Here, we present the complete mitochondrial genome of , a Common Rose butterfly from Malaysia. The sequence was generated using Illumina NovaSeq 6000 sequencing platform. The mitogenome is 15,235bp long, consisting of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two D-loop regions.

Draft Genome Sequence of Bacillus tropicus Strain UPM-CREST01, Isolated from the Bulk Paddy Soil at Kampung Gajah, Perak.

We characterized the draft genome of the potentially beneficial Bacillus tropicus strain UPM-CREST01, which was isolated from the bulk soil at a paddy cultivation area in Kampung Gajah, Perak, Malaysia. The final draft assembly of 5,252,705 bp, with a G+C content of 35.23%, was found to harbor 5,368 coding sequences, including several plant-growth-promoting genes.

Complete Genome Sequence of Salmonella enterica Bacteriophage PRF-SP1.

We characterized the complete genome sequence of the lytic Salmonella enterica bacteriophage PRF-SP1, isolated from Penang National Park, a conserved rainforest in northern Malaysia. The novel phage species from the family has a 39,966-bp double-stranded DNA (dsDNA) genome containing 49 protein-encoding genes and shares 90.96% similarity with Escherichia phage DY1.

Understanding the role of gut microbiota in the pathogenesis of schizophrenia.

Schizophrenia is a chronic mental disorder with marked symptoms of hallucination, delusion, and impaired cognitive behaviors. Although multidimensional factors have been associated with the development of schizophrenia, the principal cause of the disorder remains debatable. Microbiome involvement in the etiology of schizophrenia has been widely researched due to the advancement in sequencing technologies.

sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest.

A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1 was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39' 04.7' N 101° 17' 43.7'' E).

Complete genome sequence of Sphingomonas paucimobilis AIMST S2, a xenobiotic-degrading bacterium.

Complete genomes of xenobiotic-degrading microorganisms provide valuable resources for researchers to understand molecular mechanisms involved in bioremediation. Despite the well-known ability of Sphingomonas paucimobilis to degrade persistent xenobiotic compounds, a complete genome sequencing is lacking for this organism. In line with this, we report the first complete genome sequence of Sphingomonas paucimobilis (strain AIMST S2), an organophosphate and hydrocarbon-degrading bacterium isolated from oil-polluted soil at Kedah, Malaysia.

Insights into microbial community structure and diversity in oil palm waste compost.

Empty fruit bunch (EFB) and palm oil mill effluent (POME) are the major wastes generated by the oil palm industry in Malaysia. The practice of EFB and POME digester sludge co-composting has shown positive results, both in mitigating otherwise environmentally damaging waste streams and producing a useful product (compost) from these streams. In this study, the bacterial ecosystems of 12-week-old EFB-POME co-compost and POME biogas sludge from Felda Maokil, Johor were analysed using 16S metagenome sequencing.

Deciduous DPSCs Ameliorate MPTP-Mediated Neurotoxicity, Sensorimotor Coordination and Olfactory Function in Parkinsonian Mice.

Parkinson's disease (PD) is a neurodegenerative disorder defined by progressive deterioration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Dental pulp stem cells (DPSCs) have been proposed to replace the degenerated dopaminergic neurons due to its inherent neurogenic and regenerative potential. However, the effective delivery and homing of DPSCs within the lesioned brain has been one of the many obstacles faced in cell-based therapy of neurodegenerative disorders.

Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis.

Background: Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by the difficulties of obtaining high quality total RNA with minimal genomic DNA contamination. With this in mind, we evaluated the performance of Phenol-free total RNA purification kit (Amresco) in comparison with TRI Reagent (MRC) and RNeasy Mini (Qiagen) for the extraction of total RNA of Pseudomonas aeruginosa which was grown in glucose-supplemented (control) and polyethylene-supplemented (growth-limiting condition) minimal medium.