Biosynthesis of tropoelastin by elastic cartilage.

Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxyproline content measured. A fraction having an approximate molecular weight of 70,000 measured by polyacrylamide gel electrophoresis had a [14C]hydroxyproline content of 9.