The Hidden Global Diversity of the Yeast Genus Carlosrosaea: A Biodiversity Databases Perspective.

Biodiversity gaps in microorganisms, such as yeasts, blur our understanding of microbial diversity, introducing biases in their biogeography, ecology, and taxonomy. The genus Carlosrosaea is a potential plant growth booster, yet it is still a little-known yeast group. Considering that databases like GBIF and GenBank are powerful tools for exploring biodiversity data, we aimed to map the geographic distribution, ecological patterns, and taxonomic potential of the genus Carlosrosaea.

Targeting genomic receptors in voided urine for confirmation of benign prostatic hyperplasia.

Objectives: The objective of this study is to validate a hypothesis that a non-invasive optical imaging assay targeting genomic VPAC receptors on malignant cells shed in voided urine will represent either benign prostatic hyperplasia (BPH) or prostatic cancer (PCa). Risk for BPH in men 50-70 years old is 50-70% and PCa is 17%. BPH and PCa can coexist in 20% of men with BPH.

Harnessing Efficient ROS Generation in Carbon Dots Derived from Methyl Red for Antimicrobial Photodynamic Therapy.

The emergence of drug-resistant pathogenic microorganisms has become a public health concern, with demand for strategies to suppress their proliferation in healthcare facilities. The present study investigates the physicochemical and antimicrobial properties of carbon dots (CD-MR) derived from the methyl red azo dye. The morphological and structural analyses reveal that such carbon dots present a significant fraction of graphitic nitrogen in their structures, providing a wide emission range.

RelB-deficient autoinflammatory pathology presents as interferonopathy, but in mice is interferon-independent.

Background: Autoimmune diseases are leading causes of ill health and morbidity and have diverse etiology. Two signaling pathways are key drivers of autoimmune pathology, interferon and nuclear factor-κB (NF-κB)/RelA, defining the 2 broad labels of interferonopathies and relopathies. Prior work has established that genetic loss of function of the NF-κB subunit RelB leads to autoimmune and inflammatory pathology in mice and humans.

NFκB pathway dysregulation due to reduced RelB expression leads to severe autoimmune disorders and declining immunity.

Background: Genetic aberrations in the NFκB pathway lead to primary immunodeficiencies with various degrees of severity. We previously demonstrated that complete ablation of the RelB transcription factor, a key component of the alternative pathway, results in an early manifested combined immunodeficiency requiring stem cell transplantation.

Objective: To study the molecular basis of a progressive severe autoimmunity and immunodeficiency in three patients.

Mutually Dependent Clustering of SynDIG4/PRRT1 and AMPA Receptor Subunits GluA1 and GluA2 in Heterologous Cells and Primary Neurons.

Regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) at synapses is a predominant mechanism for regulating synaptic strength. We identified the transmembrane protein synapse differentiation-induced gene 1 (SynDIG1; SD1) as an AMPAR interacting protein that regulates excitatory synaptic strength and AMPAR number both and The related protein SynDIG4 (SD4; also known as PRRT1) was identified in several independent proteomic screens in complex with AMPARs, suggesting that it may function as an AMPAR auxiliary factor. Here, we show that the co-expression of SD4 with GluA1 or GluA2 homomeric AMPARs in COS cells leads to a 50 or 33% increase in the mean area of AMPAR puncta, respectively.

sp. nov. and sp. nov., two yeast species associated with bromeliads.

Eight yeast isolates with an affinity to the genus were obtained from bromeliads from different locations in Brazil. Although the formation of basidia and basidiocarp were not observed, on the basis of the results of sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region, we suggest that these isolates represent two novel species of the genus . These yeasts are phylogenetically related to and .

sp. nov. a tremellomycetes yeast associated with plants from dry and rainfall tropical forests.

Plants are important reservoirs of described and undescribed species of yeast. During a study of yeasts associated with bromeliads from the Northeast region of Brazil (collected in 2013-2017), analysis of the D1/D2 domain of the LSU rRNA and internal transcribed spacer (ITS) region identified eleven strains of yeasts as representing an unknown species of the genus . The species may have a diverse habitat in Brazil as a strain was collected from a flowering plant (Acanthaceae) in 1994.

Mass cytometry reveals species-specific differences and a new level of complexity for immune cells in the prostate.

Chronic inflammation in the benign prostate has been associated with a higher risk of developing prostate cancer. While a range of immune lineages is found in the prostate including T cells, B cells and myeloid cells, the specific subsets of immune cells with each major lineage have not been well described. In this study, we use mass cytometry (CyTOF) to comprehensively and reproducibly profile immune cells in mouse and human prostate.

Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.

Aging is associated with loss of tissue mass and a decline in adult stem cell function in many tissues. In contrast, aging in the prostate is associated with growth-related diseases including benign prostatic hyperplasia (BPH). Surprisingly, the effects of aging on prostate epithelial cells have not been established.

Carlosrosaea hohenbergiae sp. nov. and Carlosrosaea aechmeae sp. nov., two tremellaceous yeasts isolated from bromeliads in north-eastern Brazil.

Yeast surveys associated with different bromeliads in north-eastern Brazil led to the proposal of two novel yeast species, Carlosrosaea hohenbergiae sp. nov. and Carlosrosaea aechmeae sp.

Enhanced field-of-view integral imaging display using multi-Köhler illumination.

A common drawback in 3D integral imaging displays is the appearance of pseudoimages beyond the viewing angle. These pseudoimages appear when the light rays coming from each elemental image are not passing through the corresponding microlens, and a set of barriers must be used to avoid this flipping effect. We present a pure optical arrangement based on Köhler illumination to generate these barriers thus avoiding the pseudoimages.

Morphology, cell viability, karyotype, expression of surface markers and plasticity of three human primary cell line cultures before and after the cryostorage in LN2 and GN2.

Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the culture characteristics and the loss of viability of three human primary cell line cultures (human adult dermal fibroblasts (hADFs), human adult mesenchymal stem cells (hMSCs), and primary culture of tumor cells from lung adenocarcinoma (PCTCLA)).

Three-dimensional display by smart pseudoscopic-to-orthoscopic conversion with tunable focus.

The original aim of the integral-imaging concept, reported by Gabriel Lippmann more than a century ago, is the capture of images of 3D scenes for their projection onto an autostereoscopic display. In this paper we report a new algorithm for the efficient generation of microimages for their direct projection onto an integral-imaging monitor. Like our previous algorithm, the smart pseudoscopic-to-orthoscopic conversion (SPOC) algorithm, this algorithm produces microimages ready to produce 3D display with full parallax.

Does increasing community and liquor licensees' awareness, police activity, and feedback reduce alcohol-related violent crime? A benefit-cost analysis.

Approximately half of all alcohol-related crime is violent crime associated with heavy episodic drinking. Multi-component interventions are highly acceptable to communities and may be effective in reducing alcohol-related crime generally, but their impact on alcohol-related violent crime has not been examined. This study evaluated the impact and benefit-cost of a multi-component intervention (increasing community and liquor licensees' awareness, police activity, and feedback) on crimes typically associated with alcohol-related violence.

Multispectral integral imaging acquisition and processing using a monochrome camera and a liquid crystal tunable filter.

This paper presents an acquisition system and a procedure to capture 3D scenes in different spectral bands. The acquisition system is formed by a monochrome camera, and a Liquid Crystal Tunable Filter (LCTF) that allows to acquire images at different spectral bands in the [480, 680]nm wavelength interval. The Synthetic Aperture Integral Imaging acquisition technique is used to obtain the elemental images for each wavelength.

Three-dimensional resolvability in an integral imaging system.

The concept of three-dimensional (3D) resolvability of an integral imaging system is thoroughly investigated in this research. The general concept of 3D resolution fails to describe the 3D discrimination completely. Then the concepts of the depth-resolution plane and lateral-resolution plane are introduced to show the difference between the conventional 3D spatial resolution and the newly introduced 3D resolvability.

The cost-effectiveness of tailored, postal feedback on general practitioners' prescribing of pharmacotherapies for alcohol dependence.

Aims: The aims of this study were to conduct a randomised controlled trial to evaluate the cost-effectiveness of tailored, postal feedback on general practitioners' (GPs) prescribing of acamprosate and naltrexone for alcohol dependence relative to current practice and its impact on alcohol dependence morbidity.

Methods: Rural communities in New South Wales, Australia, were randomised into experimental (N=10) and control (N=10) communities. Tailored feedback on their prescribing of alcohol pharmacotherapies was mailed to GPs from the experimental communities (N=115).

The potential cost-effectiveness of general practitioner delivered brief intervention for alcohol misuse: evidence from rural Australia.

Objective: This paper aims to model General Practitioner (GP) delivered screening and brief intervention (BI), and to identify the costs per additional risky drinker who reduces alcohol consumption to low-risk levels, relative to current practice.

Method: A decision model and nine different scenarios were developed to assess outcomes and costs of GP-delivered screening and BI on the potential number of risky drinkers who reduce their alcohol consumption to low-risk levels in 10 rural communities in New South Wales, Australia.

Findings: Based on evidence from current practice, approximately 19% of all risky drinkers visiting GPs annually would reduce alcohol consumption to low-risk levels, of which 0.

Measuring costs of alcohol harm to others: a review of the literature.

Introduction: People other than the drinker experience harmful consequences from alcohol misuse, accounting for part of the economic burden to society. Little has been done on costing harm to others.

Method: A literature review was undertaken of various databases, government publications, dissertations, conference papers and reference materials.

Metastasis to the tunica vaginalis testis from a primary mucinous tumor of the cecum.

Objectives: We review the literature about secondary testicular tumors.

Methods: We present the case of a patient with a metastatic mucus-secreting testicular tumor who presented in the emergency room with symptoms of acute scrotum 10 months after surgery for a mixed tumor of the cecum. This type of tumor is rare, and the treatment of choice is orchiectomy; nevertheless, the prognosis of such metastasis remains poor.

Median raphe cyst. Report of two cases and literature review.

Objectives: To report 2 cases of median raphe cysts, 1 in the penis and the other in the perineum.

Method: Two cases of median raphe cyst are described; the first was treated by surgery and the second required no treatment.

Results: The surgical patient experienced no complications and was asymptomatic with no recurrence at 2 years.