Publications by authors named "Hector A Baptista Gonzalez"
Background: Evaluating the ABO/RhD blood group and the direct antiglobulin Coombs test (DAT) at birth is recommended good practice, but there is variability in its universal implementation. This study aims to show the comparative results in various variables of clinical impact during the hospital stay of neonates with positive DAT compared with those with negative DAT, based on the systematic detection of the ABO/RhD group and DAT at birth.
Methods: Newborns between 2017 and 2020 in a high-risk pregnancy care hospital were included.
Background And Objectives: Computational biology analyses the theoretical tertiary structure of proteins and identifies the 'topological' differences between RhD and RhCE. Our aim was to identify the theoretical structural differences between the four isoforms of RhCE and RhD using computational biological tools.
Materials And Methods: Physicochemical profile was determined by hydrophobicity and electrostatic potential analysis.
We present a study performed on 54 unrelated subjects, with and without thalassemic features. Two primer pairs were proposed to perform Sanger sequencing of the complete gene. The bioinformatic analysis was performed taking advantage of the availability of free online tools.
View Article and Find Full Text PDFDesign of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease.
J Oral Biol Craniofac Res
May 2016
Article Synopsis
- The study aimed to develop a protocol for extracting high-quality genomic DNA from saliva samples collected from patients with periodontal disease using mouthwash.* -
- Saliva was collected from 60 patients and DNA extraction was done at 10, 20, and 30 days, with the highest DNA concentration (352 μg) achieved at 10 days.* -
- The method proved to be effective, yielding DNA of good quality and purity, making it a simple and cost-effective approach for genomic studies.*
Aims: To present the strategy of identifying the molecular variants of G6PD detected in neonatal screening (NS).
Material And Methods: We present a series of incident cases of newborns positive for G6PD deficiency detected in NS. From nuclear DNA with the methodology of real-time PCR we sought molecular G6PD variants: G202A, A376G, T968C and C563T.
Background: The prevalence of the RhD and RhCE gene alleles is related to the ethnic mixture. The aim of this report is to describe the predominant molecular mechanisms in RhD negative subjects residents from Mexico's valley according to the phenotype of RhCE.
Methods: Blood samples from RhD negative women and men were studied.
Background & Aim: Adiponectin and ghrelin are hormones that participate in hepatic lipid metabolism, and their expression in liver tissue could have important implications for nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the hepatic expression of ghrelin, adiponectin, AdipoR, and IL-6 in patients with NAFLD and normal liver.
Methods: We studied patients with clinical-pathological diagnosis of NAFLD or a normal liver.
Background And Objective: Iron overload has been associated with HFE mutations (C282Y and H63D). We investigated the association between these mutations and high serum ferritin in a sample of healthy adult men.
Design And Methods: We enrolled unrelated blood donors from three hospitals in Mexico City in a crosssectional study.
Article Synopsis
- The study investigated the prevalence of hepatitis B (HBV) and hepatitis C (HCV) infections among nurses at Medica Sur Clinic in Mexico, given their high risk of exposure.
- Among 376 nurses, 1.6% tested positive for HBV exposure (Anti-HBc), but no one was actively infected (HBsAg positive), while 0.8% were positive for HCV antibodies, indicating prior exposure.
- Risk factors identified included having tattoos and multiple sexual partners for HBV, and older transfusions for HCV, with one nurse showing a significant viral load and HCV genotype 2b, but overall seropositivity was lower than in the general population.
Objective: Investigate the association between polymorphisms in the leptin receptor gene associated with obesity and gallstone disease.
Design: We conducted a cross-sectional study, carried out at a tertiary setting.
Subjects: We enrolled 97 subjects, comprising 54 subjects with gallstones (cases) and 43 controls (without gallstones).
Article Synopsis
- - The study aimed to investigate how ghrelin, a hormone, might influence the development of gallstone disease by comparing 38 patients with gallstones to 112 healthy controls.
- - Results indicated significant differences between the two groups regarding age, body mass index (BMI), and other metabolic factors, with higher ghrelin levels linked to a lower risk of gallstone disease.
- - Overall, findings suggest that higher serum ghrelin concentrations could provide a protective effect against gallstones, as evidenced by a notable presence of ghrelin mRNA in gallbladder samples.
After the introduction of second generation ELISA and confirmatory tests clinically available, it was possible to determine that prevalence of infection with HCV was 98% among hemophiliacs exposed to factor VIII concentrates that weren't submitted to viral inactivation. Liver failure is 4.2 times more probable among patients also infected with HIV.
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