Belgian Recommendations for Analytical Verification and Validation of Immunohistochemical Tests in Laboratories of Anatomic Pathology.

Analytical verification and validation of immunohistochemical (IHC) tests and their equipment are common practices for today's anatomic pathology laboratories. Few references or guidelines are available on how this should be performed. The study of Sciensano (the Belgian national competent authority regarding licensing of medical laboratories) performed in 2016, demonstrated a significant interlaboratory variation in validation procedures of IHC tests among Belgian laboratories.

Brain Aging and Anesthesia.

Herein, the authors review the neuroanatomical and the neurophysiological aspects of the normal aging evolution based on the recent literature and briefly describe the difference between physiological and pathological brain aging, with consideration of the currently recommended anesthesia management of older patients. The population of elderly patients is growing drastically with advances in medicine that have prolonged the life span. One of the direct consequence has been a significant increase in the request for anesthesia care for older patients despite the type of surgery (cardiac vs noncardiac and mainly orthopedic).

Does Lung Compliance Optimization Through PEEP Manipulations Reduce the Incidence of Postoperative Hypoxemia in Laparoscopic Bariatric Surgery? A Randomized Trial.

Background: In obese patients (OP), the best intraoperative ventilation strategy remains to be defined. Dynamic lung compliance (C) and dead space fraction are indicators of efficient ventilation at an optimal positive end-expiratory pressure (PEEP). Herein, we investigated whether intraoperative dynamic lung compliance optimization through PEEP manipulations affects the incidence of postoperative hypoxemia (SpO < 90%) in OP undergoing laparoscopic bariatric surgery (LBS).

[Effectiveness and clinical significance of brief dynamic psychotherapy: the Duesseldorf Short-Term Dynamic Psychotherapy Project (since 1991)].

Objectives: This study evaluates the effectiveness of short-term dynamic psychotherapy for patients with psychosomatic and personality disorders (target groups) compared to those with neurotic disorders (anxiety and depression).

Methods: Using a naturalistic design, 25 sessions of the CMP/SASB model of short-term dynamic therapy were applied to 112 outpatients. Patients with neurotic disorders constituted the control group.

A nonrandomized follow-up comparison between standard axillary node dissection and sentinel node biopsy in breast cancer.

Introduction: In many countries sentinel node biopsy (SNB) has become the standard of care in breast cancer based on a large number of observational studies but without results from prospective randomized trials. The goal of our study was to evaluate the oncological safety of the SNB in breast cancer in a multicenter, nonrandomized setting with comparable groups.

Patients And Methods: Between 1996/05 and 2004/11, 2942 patients from 14 departments in Austria with unicentric, unilateral, invasive disease without neoadjuvant therapy were collected in a database.

Interaction of B-cell hybridomas with fibroblast or hepatocyte monolayers in vitro and their metastatic behaviour in vivo.

Using an in vitro monolayer assay (MIA) we analyzed the invasive behaviour of a panel of B-cell hybridomas prepared by the fusion of non-invasive, non-metastatic NSO plasmacytoma cells and normal murine B-cells. Interaction of these hybridomas with fibroblast-like monolayers consisted mostly of adhesion on top of the monolayers, whereas only a fraction of these cells penetrated through the monolayer. This is in sharp contrast with the highly invasive properties displayed by T-cell hybridomas.

Experimental analysis of the metastatic phenotype of malignant leukocytes.

The metastatic phenotype (M+) is determined by the expression of the invasive (I+) and growth (G+) phenotypes at various sites along the metastatic pathway. The G, I and M phenotypes are not confined to tumor cells and may be expressed by normal cells, such as leukocytes, under certain physiological conditions. The study of the I and M phenotypes of leukocytes may yield valuable information on common mechanisms, underlying invasion and metastasis of normal and malignant cells.

Identification of T-cell antigens expressed by metastatic T-cell hybridomas and lymphomas.

Using the murine BW5147 tumor model system, we have identified 3 MAbs that discriminate between metastatic and non-metastatic BW5147-derived T-cell hybridomas and BW5147-unrelated T-lymphomas. The 3 rat MAbs appear to recognize an identical membrane-associated sialoglycoprotein with an approximate molecular weight of 95-100 kDa. We thus defined "metastatic T-cell hybridoma" antigens (MTH-Ags) that are also expressed on normal T-lymphocytes.

Suggestive evidence that genes controlling invasion and metastasis of T-cell lymphomas are located on mouse chromosome 3.

Cell lines differing in their malignant potential have been derived from the murine BW5147 T-cell lymphosarcoma. To evaluate the involvement of chromosomal aberrations in tumor progression within this model, we have analyzed the karyotypes and the in vitro invasiveness of 13 related nonmetastatic and metastatic variants. Giemsa banding revealed the presence of several marker chromosomes, one of which was of particular importance.

Dual effects of pertussis toxin on in vitro invasive behavior of metastatic lymphoma variants.

A simple monolayer invasion assay (MIA) was recently developed using confluent fibroblastic cells as a target and variants of the BW5147 murine T-cell lymphoma as invading cells. Metastatic variants were consistently invasive in the MIA whereas non-metastatic cells were not. In this paper it is reported that pertussis toxin (PT) treatment of a highly metastatic and invasive variant caused a marked delay of invasion in the MIA at concentrations from 50 pg/ml upwards.

Syngeneic in vivo passage of the murine BW 5147 lymphoma results in the expression of a stable metastatic phenotype.

BW 5147 lymphoma cells are non-invasive tumor cells which do not generate experimental metastases following i.v. inoculation.

New instruments and products. Spectacle-mounted telescopic lenses for children.

Telescopic aids for children have received little attention in the ophthalmic literature. What has been written is anecdotal and stresses the negative aspects of mobility with spectacle mounted aids. The benefits of a telescopic system that also maintains the patient's mobility are presented in this article.