Construction and application of multi-host integrative vector system for xylose-fermenting yeast.

The xylose-fermenting yeasts (CTG clade yeasts, e.g. Scheffersomyces stipitis, Spathaspora passalidarum, Candida amazonensis and Candida jeffriesii) have the potential to be superior platforms for the conversion of lignocellulosic hydrolysate into fuel-grade ethanol and other chemical products.

[Physiological and metabolic characteristics of five xylose utilizing yeasts].

Objective: It is of great significance to improve the utilization of lignocellulosic material, the most abundant renewable resource on earth.

Methods: We studied the stress tolerance (temperature, ethanol and osmotic tolerance) of five xylose utilizing yeasts, Scheffersomyces stipitis, Candida tenuis, Spathaspora passalidarum, Candida amazonensis and Candida jeffriesii. We also tested their utilization ability of multiple carbon and nitrogen sources.