Publications by authors named "Heather S Gatehouse"
Article Synopsis
- * A review of over 150 RNAi experiments reveals that RNAi is most effective in the Saturniidae family and immunity-related genes, while epidermal gene expression is more challenging to silence.
- * The study highlights the need for more research on RNAi mechanisms in Lepidoptera and its links to immune responses, with ongoing data collection to improve understanding through a public database.
The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although subcellular localization was not proven. SI was found to exist as two forms, one coinciding with authentic BPTI on western blots and the second marginally larger due to retention of the C-terminal peptide.
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