Design, Synthesis, and Preliminary Evaluation of [Ga]Ga-NOTA-Insulin as a PET Probe in an Alzheimer's Disease Mouse Model.

Aberrant insulin signaling has been considered one of the risk factors for the development of Alzheimer's disease (AD) and has drawn considerable attention from the research community to further study its role in AD pathophysiology. Herein, we describe the development of an insulin-based novel positron emission tomography (PET) probe, [Ga]Ga-NOTA-insulin, to noninvasively study the role of insulin in AD. The developed PET probe [Ga]Ga-NOTA-insulin showed a significantly higher uptake (0.

A new solid target design for the production of Zr and radiosynthesis of high molar activity [Zr]Zr-DBN.

Due to the advent of various biologics like antibodies, proteins, cells, viruses, and extracellular vesicles as biomarkers for disease diagnosis, progression, and as therapeutics, there exists a need to have a simple and ready to use radiolabeling synthon to enable noninvasive imaging trafficking studies. Previously, we reported [Zr]zirconium--isothiocyanatobenzyl-desferrioxamine ([Zr]Zr-DBN) as a synthon for the radiolabeling of biologics to allow PET imaging of cell trafficking. In this study, we focused on improving the molar activity (A) of [Zr]Zr-DBN, by enhancing Zr production on a low-energy cyclotron and developing a new reverse phase HPLC method to purify [Zr]Zr-DBN.