Role of fibroblast growth factor receptors 1 and 2 in the metanephric mesenchyme.

To determine the importance of fibroblast growth factor receptors (fgfrs) 1 and 2 in the metanephric mesenchyme, we generated conditional knockout mice (fgfr(Mes-/-)). Fgfr1(Mes-/-) and fgfr2(Mes-/-) mice develop normal-appearing kidneys. Deletion of both receptors (fgfr1/2(Mes-/-)) results in renal aplasia.

Role of fibroblast growth factor receptors 1 and 2 in the ureteric bud.

Fibroblast growth receptors (FGFRs) consist of four signaling family members. Mice with deletions of fgfr1 or fgfr2 are embryonic lethal prior to the onset of kidney development. To determine roles of FGFR1 and FGFR2 in the ureteric bud, we used a conditional targeting approach.

Expression of somatostatin in the adult and developing mouse kidney.

Background: Somatostatin (somatotropin release inhibiting factor) (SRIF) has potent antiproliferative and antisecretory actions. In the adult kidney, somatostatin alters renal blood flow, ion transport, and water permeability. While some evidence suggests that SRIF may be produced by adult kidney tubular cells, the specific tubules generating SRIF are unknown.

Expression of somatostatin receptors 1 and 2 in the adult mouse kidney.

Systemic infusion of somatostatin in humans and rodents alters renal glomerular filtration rate, solute transport, and water clearance. Among the five different G-protein-coupled somatostatin receptors (SSTRs), SSTR1 is expressed in human kidney collecting ducts and SSTR2 is expressed in rat and human collecting ducts and glomeruli. Our laboratory recently localized SSTRs 3, 4, and 5 to mouse kidney proximal tubules.

Expression of somatostatin receptors 3, 4, and 5 in mouse kidney proximal tubules.

Background: Systemic infusion of somatostatin (SRIF) induces many physiological changes in human and rodent kidneys, including alterations in glomerular filtration, solute transport, and water clearance. Although somatostatin can bind to five different G-protein coupled receptors (SSTRs), only SSTR1 and SSTR2A proteins have been described convincingly in rat and/or human kidneys. Both are expressed primarily in collecting ducts, despite clear evidence that somatostatin also can bind to proximal tubules.