Factors associated with residual disease on re-excision in patients with ductal carcinoma in situ of the breast.

Purpose: This study examines the factors associated with the presence of residual disease at re-excision in patients with ductal carcinoma in situ of the breast.

Methods: Of 143 consecutive patients undergoing breast conservation treatment from 1990 to 1999, 90 patients (63%) underwent re-excision of the tumor bed. Grade was recorded in 70% (N = 63), size in 78% (N = 70), margin status in 96% (N = 86), volume of excision in 90% (N = 81), and extent of residual disease in 99% (N = 89).

An outbreak of Pseudomonas aeruginosa infections associated with flexible bronchoscopes.

Background: Endoscopes, including bronchoscopes, are the medical devices most frequently associated with outbreaks of nosocomial infections. We investigated an outbreak of Pseudomonas aeruginosa infections after bronchoscopic procedures.

Methods: Microbiologic results were reviewed to determine the rates of recovery of P.

Trafficking of the Ca2+-activated K+ channel, hIK1, is dependent upon a C-terminal leucine zipper.

We demonstrate that the C-terminal truncation of hIK1 results in a loss of functional channels. This could be caused by either (i) a failure of the channel to traffic to the plasma membrane or (ii) the expression of non-functional channels. To delineate among these possibilities, a hemagglutinin epitope was inserted into the extracellular loop between transmembrane domains S3 and S4.

Organ preservation therapy using induction plus concurrent chemoradiation for advanced resectable oropharyngeal carcinoma: a University of Pennsylvania Phase II Trial.

Purpose: To determine the efficacy, feasibility, and toxicity of a new regimen for locally advanced oropharyngeal carcinoma.

Patients And Methods: Patients had technically resectable stage III/IV squamous cell carcinoma of the oropharynx, exclusive of T1-2N1. Induction chemotherapy consisted of carboplatin (area under the curve formula equal to 6) and paclitaxel 200 mg/m(2) for two cycles, followed by re-evaluation.

Release of ATP during host cell killing by enteropathogenic E. coli and its role as a secretory mediator.

Enteropathogenic Escherichia coli (EPEC) causes severe, watery diarrhea in children. We investigated ATP release during EPEC-mediated killing of human cell lines and whether released adenine nucleotides function as secretory mediators. EPEC triggered a release of ATP from all human cell lines tested: HeLa, COS-7, and T84 (colon cells) as measured using a luciferase kit.

Rates of unconventional medical therapy use in patients with prostate cancer: standard history versus directed questions.

Objectives: The use of unconventional medical therapies (UMTs) in the general population has increased dramatically in the past decade. Studies have estimated that 9% to 64% of patients with cancer use UMTs, and many do not disclose this information to their physicians. This study was designed to evaluate the rates of UMT use by patients with prostate cancer revealed by standard versus directed questioning and to identify demographic markers that may predict use.

HmsT, a protein essential for expression of the haemin storage (Hms+) phenotype of Yersinia pestis.

The haemin storage (Hms) phenotype of Yersinia pestis has been shown to be involved in the blockage of fleas that is required for the transmission of plague from fleas to mammals. Previously, an operon encoding four genes, hmsHFRS, that are essential for the temperature-regulated Hms+ phenotype has been characterized. Here the isolation and characterization of a fifth gene, hmsT, that is essential for this phenotype is described.

Yersiniabactin from Yersinia pestis: biochemical characterization of the siderophore and its role in iron transport and regulation.

A siderophore-dependent iron transport system of the pathogenic yersiniae plays a role in the pathogenesis of these organisms. The structure of the yersiniabactin (Ybt) siderophore produced by Yersinia enterocolitica has been elucidated. This paper reports the purification of Ybt from Yersinia pestis and demonstrates that it has the same structure as Ybt from Y.