Publications by Heather E James

Publications by authors named "Heather E James"

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Detection of African swine fever virus by loop-mediated isothermal amplification.

Heather E James K Ebert R McGonigle Scott M Reid Neil Boonham

J Virol Methods

March 2010

A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of African swine fever virus (ASFV). This assay targets the topoisomerase II gene of ASFV and its specificity was confirmed by restriction enzyme digestion of the reaction products. The analytical sensitivity of this ASFV LAMP assay was at least 330 genome copies, and the test was able to detect representative isolates of ASFV (n=38) without cross-reacting with classical swine fever virus.

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