Effect of sulfide inhibition and organic shock loading on anaerobic biofilm reactors treating a low-temperature, high-sulfate wastewater.

To assess the long-term treatment of sulfate- and carbon-rich wastewater at low temperatures, anaerobic biofilm reactors were operated for over 900 days at 20 degrees C and fed wastewater containing lactate and sulfate. Results showed the reactors could be operated at 20 degrees C with a load rate of 1.3 g-chemical oxygen demand (COD)/L x d or less and a sulfur loading rate (SLR) of 0.

Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family.

Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and spindle pole bodies in yeast. The ciliate Tetrahymena thermophila contains at least four centrin genes as determined by sequence homology, and these have distinct localization and expression patterns.

Functional characterization of BRCA1 sequence variants using a yeast small colony phenotype assay.

Germline mutations that inactivate the tumor suppressor gene BRCA1 are associated with an increased risk of cancers of the breast and other tissues, but the functional consequence of many missense variants found in the human population is uncertain. Several predictive methods have been proposed to distinguish cancer-predisposing missense mutations from harmless polymorphisms, including a small colony phenotype (SCP) assay performed in the model organism, yeast (Saccharomyces cerevisiae). The goal of this study was to further evaluate this colony size assay.

Mutational analysis reveals a role for the C terminus of the proteasome subunit Rpt4p in spindle pole body duplication in Saccharomyces cerevisiae.

The ubiquitin/proteasome pathway plays a key role in regulating cell cycle progression. Previously, we reported that a conditional mutation in the Saccharomyces cerevisiae gene RPT4/PCS1, which encodes one of six ATPases in the proteasome 19S cap complex/regulatory particle (RP), causes failure of spindle pole body (SPB) duplication. To improve our understanding of Rpt4p, we created 58 new mutations, 53 of which convert clustered, charged residues to alanine.