Small-scale spatial analysis of intermediate and definitive hosts of Angiostrongylus cantonensis.

Background: Angiostrongyliasis is a food-borne parasitic zoonosis. Human infection is caused by infection with the third-stage larvae of Angiostrongylus cantonensis. The life cycle of A.

[Quantitative determination of biogenic amine from nervous system by UPLC MS/MS].

Objective: To establish a method for the quantitative determination of serotonin and dopamine in the nervous system of by using ultra high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC MS/MS) .

Methods: The nervous system was broken in the pure methanol solution after obtaining it by dissecting with microscope. Then, the supernatant containing the target substance after twice high speed centrifugation was got.

[Species composition and distribution of medical mollusca in Shanghai City].

Objective: To investigate the species diversity and distribution of medical mollusca in Shanghai City.

Methods: From August 2012 to October 2013, all kinds of habitats in 8 districts and counties in Shanghai City, namely Jiading, Qingpu, Baoshan, Minhang, Songjiang, Jinshan, Chongming, Pudong, were selected for the field survey according to the distribution characteristics of the river system, and all the specimens of medical mollusca in the investigation sites were collected and classified by morphological identification. Meanwhile, the species composition, habitats as well as the fauna of the medical mollusca collected were analyzed.

[Investigation on the species distribution and infection status of host snails of Angiostrongylus cantonensis in Shanghai].

Objective: To investigate the species distribution and infection status of host snails of Angiostongylus cantonensis in Shanghai.

Methods: From August 2012 to October 2014, 10 districts or counties were selected for this investigation in Shanghai, including Jiading, Qingpu, Putuo, Baoshan, Yangpu, Minhang, Songjiang, Jinshan, Chongming, and Pudong. All of the snails were examined by lung-microscopy and tissue homogenate methods.

[Comparative analysis of mitochondrial genomes of Angiostrongylus cantonensis].

Objective: To compare the diversity of mitochondrial genomes of Angiostrongylus cantonensis in the mainland of China.

Methods: According to the population genetic of A. cantonensis, seven female worms were selected to characterize the mitochondrial (MT) genomes.

The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis.

The two rodent intra-arterial nematodes, Angiostrongylus cantonensis and Angiostrongylus costaricensis, can cause human ill-health. The present study aimed to characterize and compare the mitochondrial (mt) genomes of these two species, and clarify their phylogenetic relationship and the position in the phylum Nematoda. The complete mt genomes of A.

[Multiplex PCR assay for the detection of Angiostrongylus cantonensis larvae in Pomacea canaliculata].

Objective: To establish a multiplex PCR assay for detecting Angiostrongylus cantonensis larvae in Pomacea canaliculata.

Methods: A pair of specific primers was designed based on the sequences of the small subunit rDNA of A. cantonensis (GenBank jAY295804), in combination with 16s rDNA specific primers of P.

[Survey on the focus of Angiostrongylus cantonensis in Guangdong Province].

Objective: To understand the distribution of Angiostrongylus cantonensis foci in Guangdong Province for making surveillance program.

Methods: Survey sites were chosen by strata sampling according to different geographic locations. Totally 22 survey sites were selected in four regions: East Guangdong, West Guangdong, North Guangdong and the Pearl River Delta.

Invasive snails and an emerging infectious disease: results from the first national survey on Angiostrongylus cantonensis in China.

Background: Eosinophilic meningitis (angiostrongyliasis) caused by Angiostrongylus cantonensis is emerging in mainland China. However, the distribution of A. cantonensis and its intermediate host snails, and the role of two invasive snail species in the emergence of angiostrongyliasis, are not well understood.

Angiostrongylus cantonensis: morphological and behavioral investigation within the freshwater snail Pomacea canaliculata.

An infection with Angiostrongylus cantonensis, the main causative agent for human eosinophilic encephalitis, can be acquired through the consumption of the freshwater snail Pomacea canaliculata. This snail also provides a suitable model to study the developmental morphology and behavior of A. cantonensis larvae, facilitated by the snail's distinct lung structure.

[Morphology of III stage larvae of Angiostrongylus cantonensis in Pomacea canaliculata].

Objective: To observe the morphologic characteristics of III stage larvae of Angiostrongylus cantonensis from Pomacea canaliculata.

Methods: P. canaliculata, the intermediate host snail of A.

[Enzyme histochemistry: the effect of META-Li on Oncomelania hupensis].

Objective: To explore the killing mechanism of META-Li against Oncomelania hupensis by observing the change of enzyme activity in snail tissue.

Methods: Sixty snails were divided into 2 groups. Snails in experiment group were immersed in META-Li (100 mg/L) for 2d and soft tissue was separated for frozen sections.

[A comparative study of three methods in detecting Angiostrongylus cantonensis larvae in lung tissue of Pomacea canaliculata].

Objective: To compare the efficiency of three methods, lung-microscopy, tissue homogenate and enzyme digestion in the detection of Angiostrongylus cantonensis larvae from the lungs of snails.

Methods: 60 Pomacea canalicular infected by the first-stage larvae of A. cantonensis were divided into 2 groups and the lung of each snail from the two groups was separated from the soft body.

[Development of PCR assay for detection of Angiostrongylus cantonensis in Pomacea canaliculata].

Objective: To establish a PCR assay for detecting the third-stage larvae of Angiostrongylus cantonensis in Pomacea canaliculata.

Methods: Polymerase chain reaction primers were designed by the software Lasergene, based on the specific cDNA of the third-stage larvae of A.cantonensis in Genbank.

[Study on the molluscicidal effect of META-Li against Oncomelania hupensis].

Objective: To evaluate the molluscicidal effect of the META-Li (40% META liquid formulation) against Oncomelania snails in laboratory and field.

Methods: The experiment of META-Li against the snails by spraying, immersion and climbing-inhibition test was carried out in laboratory. Spray method was performed in the field to compare with the wettable powder of 50% niclosamide ethanolamine salt.

[Experimental study on compatibility of three species of freshwater snails with Angiostrongylus cantonensis].

Objective: To compare the compatibility of three species of freshwater snails, Pomacea canaliculata, Cipangopaludina chinensis, Bellamy a aeruginosa, with Angiostrongylus cantonensis.

Methods: The snails were infected by the first-stage larvae of A. cantonensis under the same conditions.

[Studies on the growth-development and infectivity of Angiostrongylus cantonensis in dormant Pomacea canaliculata].

Objective: To study the impact of dormancy of Pomacea canaliculata on the growth-development and infectivity of Angiostrongylus cantonensis.

Methods: The intermediate host snails (P. canaliculata) were infected with the first stage larvae of A.

The effect of temperature on the development of Angiostrongylus cantonensis (Chen 1935) in Pomacea canaliculata (Lamarck 1822).

Angiostrongyliasis cantonensis, clinically presented as eosinophilic meningitis, is a snail-borne parasitic disease. We studied the effects of different temperatures on the larval development of Angiostrongylus cantonensis in the freshwater snail Pomacea canaliculata. Six groups of snails were infected and each group was cultured under different temperature conditions.

[Studies on susceptibility of Pomacea canaliculata of different developmental stages to infection with Angiostrongylus cantonensis].

Unlabelled: OBJECTIVE To study the susceptibility of Pomacea canaliculata of different developmental stages to Angiostrongylus cantonensis infection.

Methods: P. canaliculata snails breeding at laboratory were divided into four rank (I-IV) according to the weight, and infected with the first stage larvae of A.

[Analysis of cytochrome c oxidase I and cytochrome b genes of F1 in laboratory line Oncomelania hupensis hupensis].

Objective: To analyze the diversity of F1 in laboratory line Oncomelania hupensis hupensis.

Methods: Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes were amplified by polymerase chain reaction (PCR). The nucleotide sequences of the PCR products were analyzed by GENETYX-MAC software package (ver.