[Effect of puerarin on the expression of NMDA receptor in the hippocampus CA1 region after focal cerebral ischemia in rats].

Objective: To study the antagonistic action of Puerarin against the excitatory amino acid toxicity, and to further explore its brain protection mechanisms.

Methods: Focal cerebral ischemia model was set up with middle cerebral artery occlusion by intraluminal block in this study. After cerebral ischemia, Puerarin was administered at different time point.

[Protective effect of puerarin against calcium overload after focal cerebral ischemia injury in rats].

Objective: To observe the temporal and spatial changes in the distribution of Ca2+ in the rat brain following focal cerebral ischemia injury and explore the protective effect of puerarin against calcium overload.

Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion in rats. After cerebral ischemia, puerarin was administered in the rats at different time points.

[Hypoxia inducible factor-1alpha expression trends in hippocampus of rat of different ages].

Objective: To reveal the expression trends of HIF-1alpha in hippocampus of different age rats, investigate the role of HIF-1alpha in the aging process of nervous system.

Methods: The cerebral tissues was collectd from rats of different age, including 3, 18, 24, 36 months old. There were 6 rats in each age group.

Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats.

Objective: To observe the effect of puerarin on the learning-memory disorder after global cerebral ischemia-reperfusion injury in rats, and to explore its mechanism of action.

Methods: The global cerebral ischemia-reperfusion injury model was established using the modifified Pulsinelli four-vessel occlusion in Sprague-Dawley rats. Rats were intraperitoneally injected with puerarin (100 mg/kg) 1 h before ischemia and once every 6 h afterwards.