Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells.

Objectives: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma.

Methods: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells.

[Role of chromatin conformation in eukaryotic gene regulation].

Gene expression in eukaryotes is regulated at multiple levels, which involves various cis-regulatory elements and trans-acting factors at transcriptional level. In addition, DNA methylation and histone modifications also play crucial roles in epigenetic regulation of eukaryotic genes. It is pivotal for evaluating the regulation of gene expression to understand the structural properties and spatial organization of chromatin at 3-D level.